Evidence map›Paper›PMID 34709783›Full record

ArticleACS applied materials & interfaces2021

Nanoparticle-Enabled Multiplexed Electrochemical Immunoassay for Detection of Surface Proteins on Extracellular Vesicles.

Seonhwa Lee, Bruno P Crulhas, Sonja Suvakov, Stanislav V Verkhoturov, Dmitriy S Verkhoturov, Michael J Eller, Harmeet Malhi, Vesna D Garovic, Emile A Schweikert, Gulnaz Stybayeva and 1 more

Open access · greenAbstract read
In one paragraph

Article in ACS applied materials & interfaces, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.5field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 27 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. Multiplexed electrochemical assays for clinical applications.Current opinion in electrochemistry · 2023
    Article
  10. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Seonhwa LeeDepartment of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, Minesotta 55905, United States.
Bruno P CrulhasDepartment of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, Minesotta 55905, United States.
Sonja SuvakovDivision of Nephrology and Hypertension, Mayo Clinic, Rochester, Minesotta 55905, United States.
Stanislav V VerkhoturovDepartment of Chemistry, Texas A&M University, College Station, Texas 77843, United States.
Dmitriy S VerkhoturovDepartment of Chemistry, Texas A&M University, College Station, Texas 77843, United States.
Michael J EllerDepartment of Chemistry and Biochemistry, California State University Northridge, Northridge, California 91330, United States.ORCID 0000-0002-9069-2180
Harmeet MalhiDivision of Gastroenterology and Hepatology, Mayo Clinic, Rochester, Minesotta 55905, United States.
Vesna D GarovicDivision of Nephrology and Hypertension, Mayo Clinic, Rochester, Minesotta 55905, United States.
Emile A SchweikertDepartment of Chemistry, Texas A&M University, College Station, Texas 77843, United States.ORCID 0000-0003-3964-7998
Gulnaz StybayevaDepartment of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, Minesotta 55905, United States.
Alexander RevzinDepartment of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, Minesotta 55905, United States.ORCID 0000-0002-8737-3250
Mayo Clinic · USTexas A&M University · USCalifornia State University, Northridge · US

Funding

Pathobiology of Liver InjuryR01DK111378 · NIDDK · MAYO CLINIC ROCHESTER · PI MALHI, HARMEET · 2016 to 2025
$3.9M
Context rich mass spectrometry of molecular localization and cellular interactionsR01GM123757 · NIGMS · MAYO CLINIC ROCHESTER · PI REVZIN, ALEXANDER, SCHWEIKERT, EMILE ALFRED · 2017 to 2020
$2.1M
Detection of extracellular vesicles in urine for diagnosis of preeclampsiaR42HD096993 · NICHD · SERSENSE, INC. · PI STYBAYEVA, GULNAZ · 2024 to 2025
$1.6M
Point of care detection of extracellular vesicles in urine for diagnosis of preeclampsiaR41HD096993 · NICHD · SERSENSE, INC. · PI STYBAYEVA, GULNAZ · 2018 to 2018
$225k
NICHD NIH HHS R41 HD096993NICHD NIH HHS R42 HD096993NIDDK NIH HHS R01 DK111378NIGMS NIH HHS R01 GM123757
6 · The paper itself

Abstract

Extracellular vesicles (EVs) are lipid bilayer particles secreted from various cells. EVs carry molecular information of parent cells and hold considerable promise for early disease diagnostics. This paper describes a general strategy for multiplexed immunosensing of EV surface proteins, focusing on surface markers CD63, CD81, nephrin, and podocin to prove the concept. This sensing strategy entailed functionalizing gold nanoparticles (AuNPs) with two types of antibodies and then tagging with metal ions, either Pb

Indexed as

biomarkerelectrochemical biosensorsextracellular vesiclesimmunoassayssimultaneous detection

Identifiers

PMID34709783
PMCPMC11235089
OpenAlexW3209272672

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.