ArticleParasitology2022
Lurking in the water: testing eDNA metabarcoding as a tool for ecosystem-wide parasite detection.
Article in Parasitology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Long-term trends in parasite diversity and infection levels: approaches and patterns.Biological reviews of the Cambridge Philosophical Society · 2026Review
- Development of a High-Sensitive qPCR-Based Molecular Diagnosis Method for Detection ofInternational journal of molecular sciences · 2026Article
- Next-generation detection in bovine respiratory and enteric diseases: metagenomic and amplicon sequencing insights into microbial diversity.Frontiers in veterinary science · 2026Review
- Veterinary medicine in reintroduction and reinforcement of the European pond turtle Emys orbicularis: a review.BMC veterinary research · 2025Review
- Elucidation of the life cycle of the trematodeParasitology · 2025Article
- Characterizing the Helminth Community of the Mountain Gazelle (Gazella gazella Pallas, 1766) Through DNA Metabarcoding.Acta parasitologica · 2025Article
- The abundance of snail hosts mediates the effects of antagonist interactions between trematodes on the transmission of human schistosomes.Infectious diseases of poverty · 2024Article
- Comparison of qPCR and metabarcoding for environmental DNA surveillance of a freshwater parasite.Ecology and evolution · 2024Article
- Working towards a conservation plan for fish parasites: Cyprinid parasites from the south African cape fold freshwater ecoregion as a case study.International journal for parasitology. Parasites and wildlife · 2023Article
- A Call For More Ecologically And Evolutionarily Relevant Studies of Immune Costs.Evolutionary ecology · 2023Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the light of global biodiversity change and emerging disease, there is an urgent need to establish efficient monitoring programmes of parasites in aquatic ecosystems. However, parasite identification is time-consuming, requires a high degree of taxonomic expertize and in general requires lethal sampling. The use of environmental DNA methodology to identify parasites has the potential to circumvent these limitations. This study evaluates the use of eDNA metabarcoding to detect the presence of all species of nematode and platyhelminth parasites in two New Zealand lakes. We developed two novel metabarcoding primer pairs targeting a region of cytochrome oxidase I gene (COI) specific to platyhelminths and nematodes. We successfully detected parasite DNA in both lakes. Platyhelminth DNA yield was in general greater than nematode DNA yield. This most likely results from the larger biomass of the former quantified using traditional methods, or the presence of free-swimming life stages in the life cycle of many platyhelminths. By using eDNA, we did not detect all expected parasite families revealed through traditional methods, likely due to a lack of sequencing data available from public databases such as GenBank. As such, genetic resources need to include full reference sequences if parasitology is to truly harness eDNA to characterize and monitor parasite biodiversity in natural systems.
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