Evidence map›Paper›PMID 34707001›Full record

ArticleZhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences

Expression and purification of Rv2654 recombinant protein from

Fuchao Chen, Quan Zhu, Yanyan Yu, Kanglin Wan, Qizhi Luo, Ping Yu

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 86% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Fuchao ChenDepartment of Immunology, School of Basic Medical Science, Central South University, Changsha 410078. fuchaochen2008@163.com.
Quan ZhuDepartment of Immunology, School of Basic Medical Science, Central South University, Changsha 410078.
Yanyan YuClinical Laboratory, Hunan Chest Hospital, Changsha 410006.
Kanglin WanNational Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102200, China.
Qizhi LuoDepartment of Immunology, School of Basic Medical Science, Central South University, Changsha 410078. Luoqz1025@163.com.
Ping YuDepartment of Immunology, School of Basic Medical Science, Central South University, Changsha 410078.
Central South University · CNHunan Province Chest Hospital · CNNational Institute for Communicable Disease Control and Prevention · CN

Funding

the National Science and Technology Major Special Projects during the 12th Five-Year Plan Period 2012ZX10005010-003the Natural Science Foundation of Hunan Province 2020JJ4768
6 · The paper itself

Abstract

objectivesWith the decreased protective effect of Bacille Calmette-Guérin (BCG) vaccine, widespread drug-resistant strains of tuberculosis as well as the lack of effective vaccines, global morbidity and mortality of tuberculosis remains high. The purpose of this study is to evaluate the potential of

methodsIsopropyl-beta-

resultsThe recombinant protein Rv2654 was successfully induced and expressed. The ELISA data showed that the recombinant protein Rv2654 was responsive to the serum of BCG-inoculated patients or active pulmonary tuberculosis patients. In immunized mice with recombinant protein Rv2654, the expression of IFN-γ, TNF-α, IL-2, IL-4, IL-6, IL-10 and other cytokines in peripheral blood was elevated (all

conclusionsRv2654 protein could induce the cell immune responses and it has good binding ability with serum of BCG-inoculated patients and active pulmonary tuberculosis patients, suggesting that it may serve as a good candidate antigen for tuberculosis immunological prophylaxis and diagnostic vaccines.

Indexed as

Mycobacterium tuberculosisAnimalsAntigens, BacterialBCG VaccineHumansImmunity, CellularMiceRecombinant ProteinsAntigens, BacterialBCG VaccineRecombinant Proteinscellular immunityhumoral immunityMycobacterium tuberculosisRv2654 recombinant proteintuberculosis vaccine

Identifiers

PMID34707001
PMCPMC10930181
OpenAlexW3208444969

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.