ArticleScience China. Life sciences2022
A 5' tRNA-Ala-derived small RNA regulates anti-fungal defense in plants.
Article in Science China. Life sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 45 citations in OpenAlex.
- Photoperiodic patterns in miRNA-mRNA pairs and tRNA fragments revealed by time-course co-sequencing in Arabidopsis.PLoS genetics · 2026Article
- RNA regulation in plants.Science China. Life sciences · 2026Review
- Enhancing apple disease resistance via magnetic nanoparticle-mediated delivery of artificial miRNAs that suppress a tRNA-derived small RNA targeting a disease-resistant gene.Plant communications · 2026Article
- OsRNS4-mediated cross-kingdom RNA interference is inhibited byScience advances · 2026Article
- Comprehensive study of tRNA-derived fragments in plants for biotic stress responses.Functional & integrative genomics · 2025Article
- Diverse plant RNAs coatProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Review
- A Bifunctional Nuclease Promotes the Infection of Zucchini Yellow Mosaic Virus in Watermelon by Targeting P3.Plants (Basel, Switzerland) · 2024Article
- Impaired 2',3'-cyclic phosphate tRNA repair causes thermo-sensitive genic male sterility in rice.Cell research · 2024Article
- Use of CRISPR Technology in Gene Editing for Tolerance to Biotic Factors in Plants: A Systematic Review.Current issues in molecular biology · 2024Review
- An RdDM-independent function of Pol V transcripts in gene regulation and plant defence.Nature plants · 2024Article
- PBOX-sRNA-seq uncovers novel features of miRNA modification and identifies selected 5'-tRNA fragments bearing 2'-O-modification.Nucleic acids research · 2024Article
- Transcriptome analysis of iron over-accumulating Arabidopsis genotypes uncover putative novel regulators of systemic and retrograde signaling.The plant genome · 2024Article
- RNS2 is required for the biogenesis of a wounding responsive 16 nts tsRNA in Arabidopsis thaliana.Plant molecular biology · 2024Article
- Beyond Loading: Functions of Plant ARGONAUTE Proteins.International journal of molecular sciences · 2023Review
- Stress-induced tRNA fragments take action in alternative splicing in Arabidopsis.Plant physiology · 2023Article
- Systematic characterization of small RNAs associated with C. elegans Argonautes.Science China. Life sciences · 2023Article
- Beyond transcription: compelling open questions in plant RNA biology.The Plant cell · 2023Article
- Extracellular RNA: mechanisms of secretion and potential functions.Journal of experimental botany · 2023Article
- Single-base resolution mapping of 2'-O-methylation sites by an exoribonuclease-enriched chemical method.Science China. Life sciences · 2023Article
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7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Apart from their primordial role in protein synthesis, tRNAs can be cleaved to produce tRNA-derived small RNAs (tsRNAs). The biological functions of tsRNAs in plants remain largely unknown. In this study, we developed RtcB ligation-based small RNA (sRNA) sequencing, a method that captures and distinguishes between 3'-2',3'-cyclic-phosphate (cP)/phosphate (P)-terminated sRNAs and 3'-OH-terminated sRNAs, and profiled 5' tsRNAs and 5' tRNA halves in Arabidopsis thaliana. We found that Arabidopsis 5' tsRNAs and 5' tRNA halves predominantly contain a cP at the 3' end and require S-like RNase 1 (RNS1) and RNS3 for their production. One of the most abundant 5' tsRNAs, 5' tsR-Ala, by associating with AGO1, negatively regulates Cytochrome P450 71A13 (CYP71A13) expression and camalexin biosynthesis to repress anti-fungal defense. Interestingly, 5' tsR-Ala is downregulated upon fungal infection. Our study provides a global view of 5' tsRNAs and 5' tRNA halves in Arabidopsis and unravels an important role of a 5' tsRNA in regulating anti-fungal defense.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.