Evidence map›Paper›PMID 34705219›Full record

ArticleMolecular biology reports2022

Next-generation sequencing identification and multiplex RT-PCR detection for viruses infecting cigar and flue-cured tobacco.

Tao Zhou, Shidong Zhou, Yong Chen, Jun Wang, Ruina Zhang, Huan Xiang, Zihao Xia, Mengnan An, Xiuxiang Zhao, Yuanhua Wu

Abstract read
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In one paragraph

Article in Molecular biology reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Characteristic volatile product analysis of moldy cigar wrapper and filler using gas chromatography-ion mobility spectrometry.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Tao Zhou *College of Plant Protection, Shenyang Agricultural University, No.120 Dongling, Shenyang, 110866, Liaoning, People's Republic of China.
Shidong Zhou *College of Plant Protection, Shenyang Agricultural University, No.120 Dongling, Shenyang, 110866, Liaoning, People's Republic of China.
Yong ChenDeyang Company of Sichuan Provincial Tobacco Corporation, Deyang, 618400, Sichuan, People's Republic of China.
Jun WangDeyang Company of Sichuan Provincial Tobacco Corporation, Deyang, 618400, Sichuan, People's Republic of China.
Ruina ZhangDeyang Company of Sichuan Provincial Tobacco Corporation, Deyang, 618400, Sichuan, People's Republic of China.
Huan XiangDeyang Company of Sichuan Provincial Tobacco Corporation, Deyang, 618400, Sichuan, People's Republic of China.
Zihao XiaCollege of Plant Protection, Shenyang Agricultural University, No.120 Dongling, Shenyang, 110866, Liaoning, People's Republic of China.
Mengnan AnCollege of Plant Protection, Shenyang Agricultural University, No.120 Dongling, Shenyang, 110866, Liaoning, People's Republic of China.
Xiuxiang ZhaoCollege of Plant Protection, Shenyang Agricultural University, No.120 Dongling, Shenyang, 110866, Liaoning, People's Republic of China. zhaoxx0772@syau.edu.cn.
Yuanhua WuCollege of Plant Protection, Shenyang Agricultural University, No.120 Dongling, Shenyang, 110866, Liaoning, People's Republic of China. wuyh09@syau.edu.cn.

Funding

National Key R&D Program of China 2017YFD0201104Natural Science Foundation of China 32072391
6 · The paper itself

Abstract

backgroundEarly, precise and simultaneous identification of plant viruses is of great significance for preventing virus spread and reducing losses in agricultural yields. METHODS AND

resultsIn this study, the identification of plant viruses from symptomatic samples collected from a cigar tobacco planting area in Deyang and a flue-cured tobacco planting area in Luzhou city, Sichuan Province, China, was conducted by deep sequencing of small RNAs (sRNAs) through an Illumina sequencing platform, and plant virus-specific contigs were generated based on virus-derived siRNA sequences. Additionally, sequence alignment and phylogenetic analysis were performed to determine the species or strains of these viruses. A total of 27930450, 21537662 and 28194021 clean reads were generated from three pooled samples, with a total of 105 contigs mapped to the closest plant viruses with lengths ranging from 34 ~ 1720 nt. The results indicated that the major viruses were potato virus Y, Chilli veinal mottle virus, tobacco vein banding mosaic virus, tobacco mosaic virus and cucumber mosaic virus. Subsequently, a fast and sensitive multiplex reverse transcription polymerase chain reaction assay was developed for the simultaneous detection of the most frequent RNA viruses infecting cigar and flue-cured tobacco in Sichuan.

conclusionsThese results provide a theoretical basis and convenient methods for the rapid detection and control of viruses in cigar- and flue-cured tobacco.

Indexed as

CucumovirusDisease ResistanceEvolution, MolecularGene Expression ProfilingMultiplex Polymerase Chain ReactionNicotianaPhylogenyPlant LeavesPotyvirusReverse Transcriptase Polymerase Chain ReactionRNA-SeqRNA, Small UntranslatedRNA, ViralSequence AlignmentTobacco Mosaic VirusVirusesRNA, Small UntranslatedRNA, ViralCigar tobaccoDeep sequencingMultiplex RT-PCRPlant virusSynergistic infection

Identifiers

PMID34705219

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.