ArticleThe EMBO journal2022
Ontogeny and function of the circadian clock in intestinal organoids.
Article in The EMBO journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed.
- Circadian synchronization between diet and gut microbiota in modulating host's health.Gut microbes · 2026Review
- Non-invasiveSurgery open science · 2026Article
- Expression of gastrointestinal clock genes and associated hormones levels in hibernating and non-hibernating Daurian ground squirrels.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2026Article
- Review
- Chronotoxici-Plate Containing Droplet-Engineered Rhythmic Liver Organoids for Drug Toxicity Evaluation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Circadian transcriptome of pancreatic adenocarcinoma unravels chronotherapeutic targets.JCI insight · 2024Article
- Advancements in understanding bacterial enteritis pathogenesis through organoids.Molecular biology reports · 2024Review
- The emergence of circadian timekeeping in the intestine.Nature communications · 2024Article
- Monitoring Circadian Oscillations with a Bioluminescence Reporter in Organoids.Journal of visualized experiments : JoVE · 2024Article
- Core clock gene BMAL1 and RNA-binding protein MEX3A collaboratively regulate Lgr5 expression in intestinal crypt cells.Scientific reports · 2023Article
- Circadian regulation of lung repair and regeneration.JCI insight · 2023Article
- Peristalsis-Associated Mechanotransduction Drives Malignant Progression of Colorectal Cancer.Cellular and molecular bioengineering · 2023Article
- Sex Inclusion in Transcriptome Studies of Daily Rhythms.Journal of biological rhythms · 2023Article
- Article
- The circadian clock ticks in organoids.The EMBO journal · 2022Article
- Ontogeny and function of the circadian clock in intestinal organoids.The EMBO journal · 2022Article
- Modelling metabolic diseases and drug response using stem cells and organoids.Nature reviews. Endocrinology · 2022Review
- Potential Role of the Circadian Clock in the Regulation of Cancer Stem Cells and Cancer Therapy.International journal of molecular sciences · 2022Review
- Temperature synchronisation of circadian rhythms in primary human airway epithelial cells from children.BMJ open respiratory research · 2022Article
- The World of Organoids: Gastrointestinal Disease Modelling in the Age of 3R and One Health with Specific Relevance to Dogs and Cats.Animals : an open access journal from MDPI · 2022Review
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Authors and funding
20 authors.
Funding
Abstract
Circadian rhythms regulate diverse aspects of gastrointestinal physiology ranging from the composition of microbiota to motility. However, development of the intestinal circadian clock and detailed mechanisms regulating circadian physiology of the intestine remain largely unknown. In this report, we show that both pluripotent stem cell-derived human intestinal organoids engrafted into mice and patient-derived human intestinal enteroids possess circadian rhythms and demonstrate circadian phase-dependent necrotic cell death responses to Clostridium difficile toxin B (TcdB). Intriguingly, mouse and human enteroids demonstrate anti-phasic necrotic cell death responses to TcdB. RNA-Seq analysis shows that ~3-10% of the detectable transcripts are rhythmically expressed in mouse and human enteroids. Remarkably, we observe anti-phasic gene expression of Rac1, a small GTPase directly inactivated by TcdB, between mouse and human enteroids, and disruption of Rac1 abolishes clock-dependent necrotic cell death responses. Our findings uncover robust functions of circadian rhythms regulating clock-controlled genes in both mouse and human enteroids governing organism-specific, circadian phase-dependent necrotic cell death responses, and lay a foundation for human organ- and disease-specific investigation of clock functions using human organoids for translational applications.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.