Evidence map›Paper›PMID 34704263›Full record

ArticleFEBS letters2021

Mutation of the conserved Asp-Asp pair impairs the structure, function, and inhibition of CTX-M Class A β-lactamase.

M Trent Kemp, Derek A Nichols, Xiujun Zhang, Kyle Defrees, Insung Na, Adam R Renslo, Yu Chen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FEBS letters, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Directed evolution of a beta-lactamase samples a wide variety of conformational states.Protein science : a publication of the Protein Society · 2025
    Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

M Trent KempDepartment of Molecular Medicine, University of South Florida College of Medicine, Tampa, FL, USA.
Derek A NicholsDepartment of Molecular Medicine, University of South Florida College of Medicine, Tampa, FL, USA.
Xiujun ZhangDepartment of Molecular Medicine, University of South Florida College of Medicine, Tampa, FL, USA.
Kyle DefreesDepartment of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, CA, USA.
Insung NaDepartment of Molecular Medicine, University of South Florida College of Medicine, Tampa, FL, USA.
Adam R RensloDepartment of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, CA, USA.
Yu ChenDepartment of Molecular Medicine, University of South Florida College of Medicine, Tampa, FL, USA.ORCID 0000-0002-5115-3600

Funding

Evolution and inhibition of carbapenemase in beta-lactam resistanceR01AI161762 · NIAID · UNIVERSITY OF SOUTH FLORIDA · PI CHEN, YU, RENSLO, ADAM R · 2021 to 2025
$3.7M
NIAID NIH HHS R01 AI161762
6 · The paper itself

Abstract

The Asp233-Asp246 pair is highly conserved in Class A β-lactamases, which hydrolyze β-lactam antibiotics. Here, we characterize its function using CTX-M-14 β-lactamase. The D233N mutant displayed decreased activity that is substrate-dependent, with reductions in k

Indexed as

Conserved SequenceAspartic Acidbeta-Lactamase Inhibitorsbeta-LactamasesCrystallography, X-RayLigandsMutant ProteinsMutationSubstrate SpecificityTetrazoles1H-tetrazoleAspartic Acidbeta-lactamase CTX-M-14beta-Lactamase Inhibitorsbeta-LactamasesLigandsMutant ProteinsTetrazolescarboxylate pairdrug resistanceshort hydrogen bondβ-lactam antibioticsβ-lactamase

Identifiers

PMID34704263
PMCPMC8858684

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.