Evidence map›Paper›PMID 34704119›Full record

ArticlePlant cell reports2022

Establishing an efficient protoplast transient expression system for investigation of floral thermogenesis in aroids.

Haruhiko Maekawa, Miyabi Otsubo, Mitsuhiko P Sato, Tomoko Takahashi, Koichiro Mizoguchi, Daiki Koyamatsu, Takehito Inaba, Yasuko Ito-Inaba

Abstract read
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In one paragraph

Article in Plant cell reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Haruhiko Maekawa *Department of Agricultural and Environmental Sciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki, 889-2192, Japan.
Miyabi Otsubo *Department of Agricultural and Environmental Sciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki, 889-2192, Japan.
Mitsuhiko P SatoDepartment of Frontier Research and Development, Kazusa DNA Research Institute, 2-6-7 Kazusakamatari, Kisarazu, Chiba, 292-0818, Japan.ORCID http://orcid.org/0000-0001-5383-5499
Tomoko TakahashiDepartment of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University, Shimo-Okubo 255, Sakura-ku, Saitama, 338-8570, Japan.ORCID http://orcid.org/0000-0002-5094-8882
Koichiro MizoguchiDepartment of Agricultural and Environmental Sciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki, 889-2192, Japan.
Daiki KoyamatsuDepartment of Agricultural and Environmental Sciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki, 889-2192, Japan.
Takehito InabaDepartment of Agricultural and Environmental Sciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki, 889-2192, Japan.ORCID http://orcid.org/0000-0003-1750-454X
Yasuko Ito-InabaDepartment of Agricultural and Environmental Sciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki, 889-2192, Japan. ykoina@cc.miyazaki-u.ac.jp.ORCID http://orcid.org/0000-0001-9598-5196
University of Miyazaki · JPKazusa DNA Research Institute · JPSaitama University · JP

Funding

Japan Society for the Promotion of Science 18H02169Japan Society for the Promotion of Science 20H02917Japan Society for the Promotion of Science 20K21282
6 · The paper itself

Abstract

key messageFloral thermogenesis is an important reproductive strategy for attracting pollinators. We developed essential biological tools for studying floral thermogenesis using two species of thermogenic aroids, Symplocarpus renifolius and Alocasia odora. Aroids contain many species with intense heat-producing abilities in their inflorescences. Several genes have been proposed to be involved in thermogenesis of these species, but biological tools for gene functional analyses are lacking. In this study, we aimed to develop a protoplast-based transient expression (PTE) system for the study of thermogenic aroids. Initially, we focused on skunk cabbage (Symplocarpus renifolius) because of its ability to produce intense as well as durable heat. In this plant, leaf protoplasts were isolated from potted and shoot tip-cultured plants with high efficiency (ca. 1.0 × 10

Indexed as

ThermogenesisAlocasiaAraceaeBotanyFlowersProtoplastsAlocasia odoraAraceaeInflorescenceMesophyll protoplastProtein localizationSymplocarpus renifolius

Identifiers

PMID34704119
OpenAlexW3209725121

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.