ArticleSTAR protocols2021
Protocol to assess two distinct components of the nonlinear capacitance in mouse cardiomyocytes.
Xiao-Dong Zhang, Maria Cristina Perez Flores, Valeriy Timofeyev, Ebenezer N Yamoah, Nipavan Chiamvimonvat
Open access · goldAbstract read
In one paragraphArticle in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registryThe trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
3 · Its place in the literatureWho cites it
0 citing papers in PubMed, 0 citations in OpenAlex.
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4 · The recordCorrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
5 · Who and what moneyAuthors and funding
5 authors at 3 institutions in 1 country.
Xiao-Dong ZhangDivision of Cardiovascular Medicine, Department of Internal Medicine, University of California, Davis, One Shields Avenue, GBSF 6303, Davis, CA 95616, USA.
Maria Cristina Perez FloresDepartment of Physiology and Cell Biology, University of Nevada, Reno, School of Medicine, Reno, NV 89557, USA.
Valeriy TimofeyevDivision of Cardiovascular Medicine, Department of Internal Medicine, University of California, Davis, One Shields Avenue, GBSF 6303, Davis, CA 95616, USA.
Ebenezer N YamoahDepartment of Physiology and Cell Biology, University of Nevada, Reno, School of Medicine, Reno, NV 89557, USA.
Nipavan ChiamvimonvatDivision of Cardiovascular Medicine, Department of Internal Medicine, University of California, Davis, One Shields Avenue, GBSF 6303, Davis, CA 95616, USA.
University of Nevada, Reno · USVA Northern California Health Care System · USUniversity of California, Davis · US
Funding
Structural Analyses CoreP01AG051443 · NIA · UNIVERSITY OF NEVADA RENO · PI YAMOAH, EBENEZER N · 2016 to 2025
$15.5MFunctional Interactions of Cardiac Ion ChannelsR01HL085844 · NHLBI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI CHIAMVIMONVAT, NIPAVAN · 2007 to 2025
$7.3MTranslational Study of Cardiac DysfunctionR01HL085727 · NHLBI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI CHIAMVIMONVAT, NIPAVAN, SIRISH, PADMINI · 2008 to 2022
$5.7MInner ear ion channels in healthy and diseased conditionsR01DC016099 · NIDCD · UNIVERSITY OF NEVADA RENO · PI EBENEZER N YAMOAH · 2017 to 2026
$4.7MDecoding the enigma of cardiac amplificationR01HL137228 · NHLBI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI CHIAMVIMONVAT, NIPAVAN · 2017 to 2020
$2.2MCalcium-dependent functions in Hair Cells and Spiral Ganglion NeuronsR01DC015135 · NIDCD · UNIVERSITY OF NEVADA RENO · PI GRATTON, MICHAEL ANNE NMI, YAMOAH, EBENEZER N · 2016 to 2020
$2.1MRegulation of hair cell functionsR01DC015252 · NIDCD · UNIVERSITY OF NEVADA RENO · PI YAMOAH, EBENEZER N · 2017 to 2021
$2.1MFUNCTIONAL PROPERTIES OF CA PUMPS IN HAIR CELLR01DC004215 · NIDCD · UNIVERSITY OF CINCINNATI · PI YAMOAH, EBENEZER N. · 1999 to 2003
$1.0MFunctional Regulation of Cardiac Chloride HomeostasisR56HL138392 · NHLBI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI ZHANG, XIAO-DONG · 2018 to 2018
$393kBLRD VA I01 BX000576CSRD VA I01 CX001490NHLBI NIH HHS R01 HL085727NHLBI NIH HHS R01 HL085844NHLBI NIH HHS R01 HL137228NHLBI NIH HHS R56 HL138392NIA NIH HHS P01 AG051443NIDCD NIH HHS R01 DC004215NIDCD NIH HHS R01 DC015135NIDCD NIH HHS R01 DC015252NIDCD NIH HHS R01 DC016099
6 · The paper itselfAbstract
Prestin (Slc26a5) is a motor protein previously considered to be expressed exclusively in outer hair cells (OHCs) of the inner ear. However, we recently identified the functional expression of prestin in the heart. Nonlinear capacitance (NLC) measurement in OHCs is used to evaluate the signature function of prestin, which exhibits membrane potential-dependent conformational changes. Here, we describe detailed recording techniques and quantification methods for NLC to evaluate the prestin function in mouse ventricular myocytes. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021).
Indexed as
AnimalsCells, CulturedElectric CapacitanceMembrane PotentialsMiceMyocytes, CardiacPatch-Clamp TechniquesBiophysicsCell BiologyCell isolationHealth SciencesSingle Cell
Identifiers
PMID34704077
PMCPMC8521203
OpenAlexW3206277562
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LicenceCC BY-NC-ND
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