ArticleScientific reports2021
Unraveling the complexity of faba bean (Vicia faba L.) transcriptome to reveal cold-stress-responsive genes using long-read isoform sequencing technology.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed.
- Integrating genomics, multi-omics, CRISPR and speed breeding for stress-resilient vegetable legume improvement.Functional & integrative genomics · 2026Review
- Genome-wide identification, structural characterization, and gene expression analysis of the MYB transcription factor family in faba bean (Vicia faba L.).BMC plant biology · 2026Article
- Comprehensive physiological and transcriptomic insights into drought stress responses in faba bean (Vicia Faba L.).BMC plant biology · 2025Article
- A panomics-driven framework for the improvement of major food legume crops: advances, challenges, and future prospects.Horticulture research · 2025Article
- Metabolic profiling and gene expression analyses shed light on the cold adaptation mechanisms of Saposhnikovia divaricata (Turcz.) Schischk.Scientific reports · 2025Article
- A high-density linkage map and fine QTL mapping of architecture, phenology, and yield-related traits in faba bean (Frontiers in plant science · 2025Article
- Accurate Long-Read RNA Sequencing Analysis Reveals the Key Pathways and Candidate Genes under Drought Stress in the Seed Germination Stage in Faba Bean.International journal of molecular sciences · 2024Article
- Transcriptome Analysis of Stigmas ofPlants (Basel, Switzerland) · 2024Article
- AtHVA22a, a plant-specific homologue of Reep/DP1/Yop1 family proteins is involved in turnip mosaic virus propagation.Molecular plant pathology · 2024Article
- Genotype by environment interactions and phenotypic traits stability of the EUCLEG faba bean collection.Frontiers in plant science · 2024Article
- Transcriptomic analysis reveals candidate genes associated with salinity stress tolerance during the early vegetative stage in fababean genotype, Hassawi-2.Scientific reports · 2023Article
- Genotype-by-Environment Interaction Analysis for Quantity and Quality Traits in Faba Beans Using AMMI, GGE Models, and Stability Indices.Plants (Basel, Switzerland) · 2023Article
- Article
- Genetic and Metabolite Variability among Commercial Varieties and Advanced Lines ofPlants (Basel, Switzerland) · 2023Article
- Genome-wide identification and expression analysis of the HVA22 gene family in cotton and functional analysis ofFrontiers in plant science · 2023Article
- Genome-wide association mapping for root traits associated with frost tolerance in faba beans using KASP-SNP markers.Frontiers in genetics · 2022Article
- Low Temperature Stress Tolerance: An Insight Into the Omics Approaches for Legume Crops.Frontiers in plant science · 2022Review
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9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Faba bean (Vicia faba L.), a globally important grain legume providing a stable source of dietary protein, was one of the earliest plant cytogenetic models. However, the lack of draft genome annotations and unclear structural information on mRNA transcripts have impeded its genetic improvement. To address this, we sequenced faba bean leaf transcriptome using the PacBio single-molecule long-read isoform sequencing platform. We identified 28,569 nonredundant unigenes, ranging from 108 to 9669 bp, with a total length of 94.5 Mb. Many unigenes (3597, 12.5%) had 2-20 isoforms, indicating a highly complex transcriptome. Approximately 96.5% of the unigenes matched sequences in public databases. The predicted proteins and transcription factors included NB-ARC, Myb_domain, C3H, bHLH, and heat shock proteins, implying that this genome has an abundance of stress resistance genes. To validate our results, we selected WCOR413-15785, DHN2-12403, DHN2-14197, DHN2-14797, COR15-14478, and HVA22-15 unigenes from the ICE-CBF-COR pathway to analyze their expression patterns in cold-treated samples via qRT-PCR. The expression of dehydrin-related genes was induced by cold stress. The assembled data provide the first insights into the deep sequencing of full-length RNA from faba bean at the single-molecule level. This study provides an important foundation to improve gene modeling and protein prediction.
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