Evidence map›Paper›PMID 34698969›Full record

ArticleBreast cancer research and treatment2022

KIF21A regulates breast cancer aggressiveness and is prognostic of patient survival and tumor recurrence.

Anton J Lucanus, Aye Aye Thike, Xing Fei Tan, Kee Wah Lee, Shiyuan Guo, Victoria P C King, Von Bing Yap, Boon Huat Bay, Puay Hoon Tan, George W Yip

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Article in Breast cancer research and treatment, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed, 1 pooled it
–field-weighted citation impact, top 87% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 1 synthesis or guideline pooled it, 4 citations in OpenAlex.

  1. Pooled it
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 2 countries.

Anton J LucanusDepartment of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117594, Singapore.
Aye Aye ThikeDivision of Pathology, Singapore General Hospital, Singapore, 169856, Singapore.
Xing Fei TanDepartment of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117594, Singapore.
Kee Wah LeeDepartment of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117594, Singapore.
Shiyuan GuoDepartment of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117594, Singapore.
Victoria P C KingDepartment of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117594, Singapore.
Von Bing YapDepartment of Statistics and Applied Probability, National University of Singapore, Singapore, 117546, Singapore.
Boon Huat BayDepartment of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117594, Singapore.
Puay Hoon TanDivision of Pathology, Singapore General Hospital, Singapore, 169856, Singapore.
George W YipDepartment of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117594, Singapore. georgeyip@nus.edu.sg.ORCID http://orcid.org/0000-0003-0152-0510
National University of Singapore · SGSingapore General Hospital · SG

Funding

National Medical Research Council MOH-000152National Medical Research Council NMRC/CIRG/1436/2015
6 · The paper itself

Abstract

purposeInvasion of carcinoma cells into surrounding tissue affects breast cancer staging, influences choice of treatment, and impacts on patient outcome. KIF21A is a member of the kinesin superfamily that has been well-studied in congenital extraocular muscle fibrosis. However, its biological relevance in breast cancer is unknown. This study investigated the functional roles of KIF21A in this malignancy and examined its expression pattern in breast cancer tissue.

methodsThe function of KIF21A in breast carcinoma was studied in vitro by silencing its expression in breast cancer cells and examining the changes in cellular activities. Immunohistochemical staining of breast cancer tissue microarrays was performed to determine the expression patterns of KIF21A.

resultsKnocking down the expression of KIF21A using siRNA in MDA-MB-231 and MCF7 human breast cancer cells resulted in significant decreases in tumor cell migration and invasiveness. This was associated with reduced Patched 1 expression and F-actin microfilaments. Additionally, the number of focal adhesion kinase- and paxillin-associated focal adhesions was increased. Immunohistochemical staining of breast cancer tissue microarrays showed that KIF21A was expressed in both the cytoplasmic and nuclear compartments of carcinoma cells. Predominance of cytoplasmic KIF21A was significantly associated with larger tumors and high grade cancer, and prognostic of cause-specific overall patient survival and breast cancer recurrence.

conclusionThe data demonstrates that KIF21A is an important regulator of breast cancer aggressiveness and may be useful in refining prognostication of this malignant disease.

Indexed as

Breast NeoplasmsKinesinsCytoplasmFemaleHumansNeoplasm Recurrence, LocalPrognosisKIF21A protein, humanKinesinsBreast cancerCancer invasionCancer recurrenceDisease-free survivalKIF21AOverall survival

Identifiers

PMID34698969
OpenAlexW3209609193

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.