Evidence map›Paper›PMID 34698238›Full record

ArticleMethods and protocols2021

SARS-CoV-2 Multi-Antigen Serology Assay.

Ramin Mazhari, Shazia Ruybal-Pesántez, Fiona Angrisano, Nicholas Kiernan-Walker, Stephanie Hyslop, Rhea J Longley, Caitlin Bourke, Catherine Chen, Deborah A Williamson, Leanne J Robinson and 2 more

Abstract read
In one paragraph

Article in Methods and protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Observational
  2. Article
  3. Article
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  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Ramin MazhariWalter and Eliza Hall Institute of Medical Research, Melbourne, VIC 3052, Australia.ORCID 0000-0001-5057-5994
Shazia Ruybal-PesántezWalter and Eliza Hall Institute of Medical Research, Melbourne, VIC 3052, Australia.
Fiona AngrisanoVector-Borne Diseases and Tropical Public Health, Burnet Institute, Melbourne, VIC 3004, Australia.
Nicholas Kiernan-WalkerWalter and Eliza Hall Institute of Medical Research, Melbourne, VIC 3052, Australia.
Stephanie HyslopWalter and Eliza Hall Institute of Medical Research, Melbourne, VIC 3052, Australia.
Rhea J LongleyWalter and Eliza Hall Institute of Medical Research, Melbourne, VIC 3052, Australia.ORCID 0000-0002-4510-5602
Caitlin BourkeWalter and Eliza Hall Institute of Medical Research, Melbourne, VIC 3052, Australia.
Catherine ChenWalter and Eliza Hall Institute of Medical Research, Melbourne, VIC 3052, Australia.
Deborah A WilliamsonRoyal Melbourne Hospital, Melbourne, VIC 3052, Australia.
Leanne J RobinsonWalter and Eliza Hall Institute of Medical Research, Melbourne, VIC 3052, Australia.
Ivo MuellerWalter and Eliza Hall Institute of Medical Research, Melbourne, VIC 3052, Australia.
Emily M ErikssonWalter and Eliza Hall Institute of Medical Research, Melbourne, VIC 3052, Australia.ORCID 0000-0002-7851-973X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Serology tests are extremely useful for assessing whether a person has been infected with a pathogen. Since the onset of the COVID-19 pandemic, measurement of anti-SARS-CoV-2-specific antibodies has been considered an essential tool in identifying seropositive individuals and thereby understanding the extent of transmission in communities. The Luminex system is a bead-based technology that has the capacity to assess multiple antigens simultaneously using very low sample volumes and is ideal for high-throughput studies. We have adapted this technology to develop a COVID-19 multi-antigen serological assay. This protocol described here carefully outlines recommended steps to optimize and establish this method for COVID-19-specific antibody measurement in plasma and in saliva. However, the protocol can easily be customized and thus the assay is broadly applicable to measure antibodies to other pathogens.

Indexed as

COVID-19LuminexSARS-CoV-2serology

Identifiers

PMID34698238
PMCPMC8544427

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.