Evidence map›Paper›PMID 34698227›Full record

ArticleMethods and protocols2021

Strategies to Investigate Membrane Damage, Nucleoid Condensation, and RNase Activity of Bacterial Toxin-Antitoxin Systems.

Stefano Maggi, Alberto Ferrari, Korotoum Yabre, Aleksandra Anna Bonini, Claudio Rivetti, Claudia Folli

Abstract read
In one paragraph

Article in Methods and protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Stefano MaggiDepartment of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, 43124 Parma, Italy.ORCID 0000-0003-4853-5840
Alberto FerrariDepartment of Food and Drug, University of Parma, 43124 Parma, Italy.
Korotoum YabreDepartment of Food and Drug, University of Parma, 43124 Parma, Italy.
Aleksandra Anna BoniniDepartment of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, 43124 Parma, Italy.
Claudio RivettiDepartment of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, 43124 Parma, Italy.ORCID 0000-0003-3775-6779
Claudia FolliDepartment of Food and Drug, University of Parma, 43124 Parma, Italy.ORCID 0000-0002-7187-0180

Funding

Ministero degli Affari Esteri e della Cooperazione Internazionale MAE0057459Ministero dell'Istruzione, dell'Università e della Ricerca Dipartimenti di Eccellenza (DM 11/05/2017 no. 262)
6 · The paper itself

Abstract

A large number of bacterial toxin-antitoxin (TA) systems have been identified so far and different experimental approaches have been explored to investigate their activity and regulation both in vivo and in vitro. Nonetheless, a common feature of these methods is represented by the difficulty in cell transformation, culturing, and stability of the transformants, due to the expression of highly toxic proteins. Recently, in dealing with the type I Lpt/RNAII and the type II YafQ/DinJ TA systems, we encountered several of these problems that urged us to optimize methodological strategies to study the phenotype of recombinant

Indexed as

atomic force microscopyinhibition growth assaysmembrane-associated toxinsribonuclease toxinssingle-cell fluorescence microscopytoxin–antitoxin systems

Identifiers

PMID34698227
PMCPMC8544347

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.