ArticleeLife2021
Electron cryo-tomography reveals the subcellular architecture of growing axons in human brain organoids.
Article in eLife, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
30 citing papers in PubMed, 45 citations in OpenAlex.
- Spatial confinement shapes organelle architecture and remodeling in axons.bioRxiv : the preprint server for biology · 2026Article
- Microtubules in the axon are GDP bound but adopt a stable GTP-like expanded state.Nature structural & molecular biology · 2026Article
- The diverse forms and roles of the neuronal endoplasmic reticulum.Nature reviews. Neuroscience · 2026Review
- Volume electron microscopy in axon regeneration research: insights into mitochondria, endoplasmic reticulum, and membrane contacts.Frontiers in neural circuits · 2026Review
- Organoids in respiratory virus research: advances and perspectives.Molecular biomedicine · 2025Review
- Tubular ER dysfunction in neurodegenerative diseases.Neurobiology of disease · 2025Review
- Spatially patterned cytoskeletal organization shapes astrocyte branch complexity.bioRxiv : the preprint server for biology · 2025Article
- Advancing Cryo-EM and Cryo-ET through Innovation in Sample Carriers: A Perspective.Analytical chemistry · 2025Review
- Emerging Approaches for Studying Lipid Dynamics, Metabolism, and Interactions in Cells.Annual review of biochemistry · 2025Review
- Cryo-ET suggests tubulin chaperones form a subset of microtubule lumenal particles with a role in maintaining neuronal microtubules.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- CryoVesNet: A dedicated framework for synaptic vesicle segmentation in cryo-electron tomograms.The Journal of cell biology · 2025Article
- Recent advances in correlative cryo-light and electron microscopy.Current opinion in structural biology · 2024Review
- Nonvesicular lipid transfer drives myelin growth in the central nervous system.Nature communications · 2024Article
- The Endoplasmic Reticulum and Its Contacts: Emerging Roles in Axon Development, Neurotransmission, and Degeneration.The Neuroscientist : a review journal bringing neurobiology, neurology and psychiatry · 2024Review
- Serialized on-grid lift-in sectioning for tomography (SOLIST) enables a biopsy at the nanoscale.Nature methods · 2024Article
- CryoET reveals actin filaments within platelet microtubules.Nature communications · 2024Article
- Molecular cartography within axons.Current opinion in cell biology · 2024Review
- High-confidence 3D template matching for cryo-electron tomography.Nature communications · 2024Article
- Human cerebral organoids: cellular composition and subcellular morphological features.Frontiers in cellular neuroscience · 2024Article
- Handling Difficult Cryo-ET Samples: A Study with Primary Neurons from Drosophila melanogaster.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2023Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 3 countries.
Funding
Abstract
During brain development, axons must extend over great distances in a relatively short amount of time. How the subcellular architecture of the growing axon sustains the requirements for such rapid build-up of cellular constituents has remained elusive. Human axons have been particularly poorly accessible to imaging at high resolution in a near-native context. Here, we present a method that combines cryo-correlative light microscopy and electron tomography with human cerebral organoid technology to visualize growing axon tracts. Our data reveal a wealth of structural details on the arrangement of macromolecules, cytoskeletal components, and organelles in elongating axon shafts. In particular, the intricate shape of the endoplasmic reticulum is consistent with its role in fulfilling the high demand for lipid biosynthesis to support growth. Furthermore, the scarcity of ribosomes within the growing shaft suggests limited translational competence during expansion of this compartment. These findings establish our approach as a powerful resource for investigating the ultrastructure of defined neuronal compartments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.