ArticleAnalytical chemistry2021
Hybrid Antibody-Aptamer Assay for Detection of Tetrodotoxin in Pufferfish.
Article in Analytical chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 44 citations in OpenAlex.
- SELEX-Based Aptamer Technologies for Toxin Analysis: Screening, Optimization, and Computational Assisted Design.Toxins · 2026Review
- Nanozyme-Based Colorimetric Assay on a Magnetic Microfluidic Platform for Integrated Detection of TTX.Biosensors · 2026Article
- Preliminary Development of DNA Aptamer Quantum Dot-Based Competitive Lateral Flow Assays for Saxitoxin and Tetrodotoxin.Journal of fluorescence · 2025Article
- Tetrodotoxin: The State-of-the-Art Progress in Characterization, Detection, Biosynthesis, and Transport Enrichment.Marine drugs · 2024Review
- Article
- Novel enzyme-linked aptamer-antibody sandwich assay and hybrid lateral flow strip for SARS-CoV-2 detection.Journal of nanobiotechnology · 2024Article
- Tetrodotoxin and the state-of-the-art progress of its associated analytical methods.Frontiers in microbiology · 2024Review
- Recent research progress in tetrodotoxin detection and quantitative analysis methods.Frontiers in chemistry · 2024Review
- A label-free ratiometric fluorescent aptasensor based on a peroxidase-mimetic multifunctional ZrFe-MOF for the determination of tetrodotoxin.Mikrochimica acta · 2023Article
- Multi-aptamer-mediated hairpin allosteric and aptamer-assisted CRISPR system for detection of S. pneumoniae and S. aureus.Mikrochimica acta · 2023Article
- Detection of Ciguatoxins and Tetrodotoxins in Seafood with Biosensors and Other Smart Bioanalytical Systems.Foods (Basel, Switzerland) · 2023Review
- Sensitive and rapid detection of tetrodotoxin based on gold nanoflower-and latex microsphere-labeled monoclonal antibodies.Frontiers in bioengineering and biotechnology · 2023Article
- Capture-SELEX: Selection Strategy, Aptamer Identification, and Biosensing Application.Biosensors · 2022Review
- Programmed Aptamer Screening, Characterization, and Rapid Detection for α-Conotoxin MI.Toxins · 2022Article
- Sandwich capture ultrasensitive sensor based on biohybrid interface for the detection of Cronobacter sakazakii.Applied microbiology and biotechnology · 2022Article
- An Updated Review of Tetrodotoxin and Its Peculiarities.Marine drugs · 2022Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The marine toxin tetrodotoxin (TTX) poses a great risk to public health safety due to its severe paralytic effects after ingestion. Seafood poisoning caused by the consumption of contaminated marine species like pufferfish due to its expansion to nonendemic areas has increased the need for fast and reliable detection of the toxin to effectively implement prevention strategies. Liquid chromatography-mass spectrometry is considered the most accurate method, although competitive immunoassays have also been reported. In this work, we sought to develop an aptamer-based assay for the rapid, sensitive, and cost-effective detection of TTX in pufferfish. Using capture-SELEX combined with next-generation sequencing, aptamers were identified, and their binding properties were evaluated. Finally, a highly sensitive and user-friendly hybrid antibody-aptamer sandwich assay was developed with superior performance compared to several assays reported in the literature and commercial immunoassay kits. The assay was successfully applied to the quantification of TTX in pufferfish extracts, and the results obtained correlated very well with a competitive magnetic bead-based immunoassay performed in parallel for comparison. This is one of the very few works reported in the literature of such hybrid assays for small-molecule analytes whose compatibility with field samples is also demonstrated.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.