Evidence map›Paper›PMID 34697449›Full record

ArticleOncogene2022

HOTAIR/Sp1/miR-199a critically regulates cancer stemness and malignant progression of cutaneous squamous cell carcinoma.

Jia Chen, Shu-Fen Hou, Feng-Jie Tang, Dai-Song Liu, Zi-Zi Chen, Hong-Lian Zhang, Shao-Hua Wang

Abstract read
PubMed Publisher
In one paragraph

Article in Oncogene, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
1.7field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 26 citations in OpenAlex.

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  15. Specificity Proteins (Sp) and Cancer.International journal of molecular sciences · 2023
    Review
  16. LINC00467: an oncogenic long noncoding RNA.Cancer cell international · 2022
    Review
  17. Article
  18. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Jia ChenDepartment of Plastic Surgery, The Third Xiangya Hospital of Central South University, Changsha, 410013, Hunan, PR China.
Shu-Fen HouDepartment of Plastic Surgery, The Third Xiangya Hospital of Central South University, Changsha, 410013, Hunan, PR China.
Feng-Jie TangDepartment of Plastic Surgery, The Third Xiangya Hospital of Central South University, Changsha, 410013, Hunan, PR China.
Dai-Song LiuDepartment of Plastic Surgery, The Third Xiangya Hospital of Central South University, Changsha, 410013, Hunan, PR China.
Zi-Zi ChenDepartment of Plastic Surgery, The Third Xiangya Hospital of Central South University, Changsha, 410013, Hunan, PR China.
Hong-Lian ZhangDepartment of Plastic Surgery, The Third Xiangya Hospital of Central South University, Changsha, 410013, Hunan, PR China.
Shao-Hua WangDepartment of Plastic Surgery, The Third Xiangya Hospital of Central South University, Changsha, 410013, Hunan, PR China. shaahww789@163.com.ORCID http://orcid.org/0000-0002-3863-0695
Central South University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The long non-coding RNA (lncRNA), HOX antisense intergenic RNA (HOTAIR) is a well-characterized oncogene in multiple human cancers, but not in cutaneous squamous cell carcinoma (CSCC). In this study, we focused on investigating the potential role of HOTAIR in stemness of CSCC. By measuring its expression using RT-qPCR in CSCC vs. normal tissues, as well as in CSCC cell lines A431 or SCC13, A431- or SCC13-derived CSCC stem cells (CSCSCs), and normal skin fibroblasts (HSFs), we detected higher expression of HOTAIR in CSCC than in normal tissues, in recurrent than in non-recurrent CSCC tissues, in CSCCs and CSCSCs than in HSFs, and particularly, in CSCSCs than in CSCCs. Kaplan-Meier analysis suggested that higher expression of HOTAIR was positively correlated with worse overall survival of CSCC patients. Functional assays on colony formation, EdU incorporation, sphere formation, western blot on stem-cell biomarkers, and in vivo models showed that HOTAIR was essential in maintaining multiple stem cell phenotypes of CSCSCs in vitro and in vivo xenograft growth as well as metastasis. Mechanistically, HOTAIR directly interacted with and up-regulated Sp1. Sp1 then induced DNMT1-mediated promoter methylation and direct transcriptional repression of miR-199a-5p. Targeting Sp1 or DNMT1 further boosted the in vivo anti-tumor and anti-metastasis activities of targeting HOTAIR. In conclusion, HOTAIR, by up-regulating Sp1 and targeting miR-199a, promotes stemness and progression of CSCC. Targeting HOTAIR, Sp1 or the underlying mechanisms may thus benefit CSCC treatment.

Indexed as

AnimalsCarcinoma, Squamous CellDisease ProgressionHumansMiceMice, NudeMicroRNAsNeoplastic Stem CellsRNA, Long NoncodingSkin NeoplasmsSurvival AnalysisHOTAIR long untranslated RNA, humanMicroRNAsMirn199 microRNA, mouseRNA, Long Noncoding

Identifiers

PMID34697449
OpenAlexW3208475950

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.