ArticleCommunications biology2021
Intravital imaging strategy FlyVAB reveals the dependence of Drosophila enteroblast differentiation on the local physiology.
Article in Communications biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 14 citations in OpenAlex.
- Reevaluation of whether histones are asymmetrically segregated during asymmetric divisions of stem cells inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Age mosaic of gut epithelial cells prevents aging.Nature communications · 2025Article
- Bellymount-pulsed tracking: a novel approach for real-time in vivo imaging of Drosophila abdominal tissues.G3 (Bethesda, Md.) · 2025Article
- Drosophila Intestine as a Model to Study Tumors.Advances in experimental medicine and biology · 2025Review
- Bellymount-Pulsed Tracking: A Novel Approach for Real-Time In vivo Imaging ofbioRxiv : the preprint server for biology · 2024Article
- Dietary L-Glu sensing by enteroendocrine cells adjusts food intake via modulating gut PYY/NPF secretion.Nature communications · 2024Article
- Apical-basal polarity precisely determines intestinal stem cell number by regulating Prospero threshold.Cell reports · 2023Article
- MicroRNA mediated regulation of the onset of enteroblast differentiation in the Drosophila adult intestine.Cell reports · 2022Article
- De novo apical domain formation inside theeLife · 2022Article
- Advances and challenges in intravital imaging of craniofacial and dental progenitor cells.Genesis (New York, N.Y. : 2000) · 2022Review
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Aging or injury in Drosophila intestine promotes intestinal stem cell (ISC) proliferation and enteroblast (EB) differentiation. However, the manner the local physiology couples with dynamic EB differentiation assessed by traditional lineage tracing method is still vague. Therefore, we developed a 3D-printed platform "FlyVAB" for intravital imaging strategy that enables the visualization of the Drosophila posterior midgut at a single cell level across the ventral abdomen cuticle. Using ISCs in young and healthy midgut and enteroendocrine cells in age-associated hyperplastic midgut as reference coordinates, we traced ISC-EB-enterocyte lineages with Notch signaling reporter for multiple days. Our results reveal a "differentiation-poised" EB status correlated with slow ISC divisions and a "differentiation-activated" EB status correlated with ISC hyperplasia and rapid EB to enterocyte differentiation. Our FlyVAB imaging strategy opens the door to long-time intravital imaging of intestinal epithelium.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.