ArticleScientific reports2021
Optimization of RNA extraction methods from human metabolic tissue samples of the COMET biobank.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
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- Optimized methods for obtaining sequencing-quality RNA from blubber of free-ranging cetaceans collected under field conditions.Conservation physiology · 2026Article
- Evaluating the utility of storage solutions to preserve nucleic acid integrity at ambient temperature in South Africa.African journal of laboratory medicine · 2026Article
- Developing an efficient protocol for RNA extraction from Morelet's crocodile caudal scute biopsies.MethodsX · 2025Article
- Rapid, equipment-free extraction of DNA from skin biopsies for point-of-care diagnostics.Scientific reports · 2024Article
- Development and validation of most efficient RNA isolation method from buffalo bull spermatozoa.Molecular biology reports · 2023Article
- A Comparative Study on the Effect of Euthanasia Methods and Sample Storage Conditions on RNA Yield and Quality in Porcine Tissues.Animals : an open access journal from MDPI · 2023Article
- An optimized method for high-quality RNA extraction from distinctive intrinsic laryngeal muscles in the rat model.Scientific reports · 2022Article
- Does Childhood Obesity Trigger Neuroinflammation?Biomedicines · 2022Article
- Development and characterization of three cell culture systems to investigate the relationship between primary bone marrow adipocytes and myeloma cells.Frontiers in oncology · 2022Article
- Addressing the quality challenge of a human biospecimen biobank through the creation of a quality management system.PloS one · 2022Article
- Progress Toward a Multiomic Understanding of Traumatic Brain Injury: A Review.Biomarker insights · 2022Review
Corrections and comments
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Authors and funding
12 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Constitution of biobank of human tissues requires careful handling and storage of biological material, to guarantee the quality of samples. Tissue preparation is also critical for further applications such as transcriptomic profiling. In this study, our aim was to evaluate the impact of different disruption techniques (FastPrep-24 instrument, GentleMACS dissociator, and syringe/needle) and homogenizing buffers (RLT versus QIAzol) on RNA purity and quality of metabolic tissues (adipose tissues, liver and skeletal muscle) present in the COMET Biobank. For all homogenization methods used and tissue types, the A260/280 ratios reached values ≥ 1.8, which are in the range of what is found in human tissues and cell lines, while the A260/230 ratios were however ≤ 1.8, with the lowest value obtained with GentleMACS Dissociator. In addition, GentleMACS Dissociator combined with QIAzol reagent gave the highest RIN value and 28S/18S ratio for all tissues tested, except for muscle. Performing RT-qPCR, Ct values for different housekeeping genes can be influenced by extraction methods and RNA quality of samples. In conclusion, we have demonstrated that different disruption techniques and homogenizing buffers impact the purity and some quality markers of RNA, and can also impact quantification of mRNAs by RT-qPCR in human metabolic tissues.
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