Evidence map›Paper›PMID 34696836›Full record

ReviewThe Enzymes2021

Watching the bacterial RNA polymerase transcription reaction by time-dependent soak-trigger-freeze X-ray crystallography.

Yeonoh Shin, Katsuhiko S Murakami

Open access · greenAbstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in The Enzymes, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.0field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Yeonoh ShinDepartment of Biochemistry and Molecular Biology, The Center for RNA Molecular Biology, The Pennsylvania State University, University Park, PA, United States.
Katsuhiko S MurakamiDepartment of Biochemistry and Molecular Biology, The Center for RNA Molecular Biology, The Pennsylvania State University, University Park, PA, United States. Electronic address: kum14@psu.edu.
Pennsylvania State University · US

Funding

Structural study of direct associations between cellular RNA polymerase and regulatory factors during the transcription cycleR35GM131860 · NIGMS · PENNSYLVANIA STATE UNIVERSITY, THE · PI MURAKAMI, KATSUHIKO · 2019 to 2023
$2.2M
NIGMS NIH HHS R35 GM131860
6 · The paper itself

Abstract

RNA polymerase (RNAP) is the central enzyme of gene expression, which transcribes DNA to RNA. All cellular organisms synthesize RNA with highly conserved multi-subunit DNA-dependent RNAPs, except mitochondrial RNA transcription, which is carried out by a single-subunit RNAP. Over 60 years of extensive research has elucidated the structures and functions of cellular RNAPs. In this review, we introduce a brief structural feature of bacterial RNAP, the most well characterized model enzyme, and a novel experimental approach known as "Time-dependent soak-trigger-freeze X-ray crystallography" which can be used to observe the RNA synthesis reaction at atomic resolution in real time. This principle methodology can be used for elucidating fundamental mechanisms of cellular RNAP transcription.

Indexed as

DNA-Directed RNA PolymerasesTranscription, GeneticCrystallography, X-RayRNA, BacterialDNA-Directed RNA PolymerasesRNA, BacterialRNARNA polymeraseStructural biologyTime-dependent soak-trigger-freeze X-ray crystallographyTranscription

Identifiers

PMID34696836
PMCPMC8965730
OpenAlexW3209386467

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.