ReviewViruses2021
Best Molecular Tools to Investigate Coronavirus Diversity in Mammals: A Comparison.
Review in Viruses, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 26 citations in OpenAlex.
- First Detection of Porcine Hemagglutinating Encephalomyelitis Virus (PHEV) in Wild Boars (Veterinary sciences · 2026Article
- Sequencing of Betacoronavirus erinacei from faeces of pet hedgehogs demonstrates a continuity of MERS-CoV like viruses in European and Eurasian hedgehog species.One health (Amsterdam, Netherlands) · 2026Article
- Detection and complete genome characterisation of bat coronaviruses from Ghana.Archives of virology · 2026Article
- Coronavirus diversity and SARS-CoV-2 exposure at the wildlife-human interface in Northern Italy.Virology journal · 2026Article
- Surveillance of Swine Coronaviruses in Hungarian Herds with a Newly Established Pan-Coronavirus RT-PCR System.Animals : an open access journal from MDPI · 2026Article
- Monitoring the health of wolves (Canis lupus): Integrating conservation and public health.PloS one · 2026Article
- Prevalence and genetic characterization of diarrhea viruses among cattle in Guangdong, China.BMC veterinary research · 2025Article
- Metagenomic analysis of viral diversity in Portuguese bats.Veterinary research communications · 2025Article
- Cross-neutralising antibodies to SARS-CoV-2 in sera from straw-coloured fruit bats (Eidolon helvum) in Nigeria.BMC veterinary research · 2025Article
- Article
- Molecular Surveillance for Potential Zoonotic Pathogens in Troglophilus Bats: Detection and Molecular Characterization of Bat Coronaviruses in Southern Italy.Pathogens (Basel, Switzerland) · 2025Article
- Climate Change-Driven Heatwaves Pose Lethal Risks to Newborn Forest Bats.Ecology and evolution · 2025Article
- Comparative Analysis of Quan and Watanabe Pan-Coronavirus Assays for Bat Coronavirus Diversity in Sarawak, East Malaysia.Journal of medical virology · 2025Article
- A multi-disciplinary approach to identify spillover interfaces of bat coronaviruses to pig farms in Italy.PloS one · 2025Article
- Genomic characterization and cross-species transmission potential of hedgehog coronavirus.One health (Amsterdam, Netherlands) · 2024Article
- Canine Distemper Virus in Sardinia, Italy: Detection and Phylogenetic Analysis in Foxes.Animals : an open access journal from MDPI · 2024Article
- Article
- Comparative Performance in the Detection of Four Coronavirus Genera from Human, Animal, and Environmental Specimens.Viruses · 2024Article
- Presence ofViruses · 2024Article
- First Report ofViruses · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Coronaviruses (CoVs) are widespread and highly diversified in wildlife and domestic mammals and can emerge as zoonotic or epizootic pathogens and consequently host shift from these reservoirs, highlighting the importance of veterinary surveillance. All genera can be found in mammals, with α and β showing the highest frequency and diversification. The aims of this study were to review the literature for features of CoV surveillance in animals, to test widely used molecular protocols, and to identify the most effective one in terms of spectrum and sensitivity. We combined a literature review with analyses in silico and in vitro using viral strains and archive field samples. We found that most protocols defined as pan-coronavirus are strongly biased towards α- and β-CoVs and show medium-low sensitivity. The best results were observed using our new protocol, showing LoD 100 PFU/mL for SARS-CoV-2, 50 TCID
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.