Evidence map›Paper›PMID 34696329›Full record

ArticleViruses2021

Monoclonal Antibodies to S and N SARS-CoV-2 Proteins as Probes to Assess Structural and Antigenic Properties of Coronaviruses.

Rinki Kumar, Neil D Christensen, Rebecca J Kaddis Maldonado, Maria C Bewley, Alexandria Ostman, Malgorzata Sudol, Eunice C Chen, Natalie W Buchkovich, Abhinay Gontu, Meera Surendran Nair and 9 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.3field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors at 2 institutions in 1 country.

Rinki KumarDepartment of Microbiology & Immunology, Penn State College of Medicine, Hershey, PA 17033, USA.
Neil D ChristensenDepartment of Pathology, Penn State College of Medicine, Hershey, PA 17033, USA.
Rebecca J Kaddis MaldonadoDepartment of Medicine, Penn State College of Medicine, Hershey, PA 17033, USA.
Maria C BewleyDepartment of Biochemistry & Molecular Biology, Penn State College of Medicine, Hershey, PA 17033, USA.ORCID 0000-0001-7097-0123
Alexandria OstmanDepartment of Microbiology & Immunology, Penn State College of Medicine, Hershey, PA 17033, USA.
Malgorzata SudolDepartment of Medicine, Penn State College of Medicine, Hershey, PA 17033, USA.
Eunice C ChenDepartment of Medicine, Penn State College of Medicine, Hershey, PA 17033, USA.
Natalie W BuchkovichDepartment of Microbiology & Immunology, Penn State College of Medicine, Hershey, PA 17033, USA.
Abhinay GontuDepartment of Veterinary and Biomedical Sciences, Penn State University, University Park, PA 16802, USA.
Meera Surendran NairDepartment of Veterinary and Biomedical Sciences, Penn State University, University Park, PA 16802, USA.ORCID 0000-0003-4686-8414
Ruth H NisslyHuck Institutes of the Life Sciences, Penn State University, University Park, PA 16802, USA.ORCID 0000-0002-3102-7447
Allen M MinnsHuck Institutes of the Life Sciences, Penn State University, University Park, PA 16802, USA.
Vivek KapurHuck Institutes of the Life Sciences, Penn State University, University Park, PA 16802, USA.
Randall RossiHuck Institutes of the Life Sciences, Penn State University, University Park, PA 16802, USA.
Suresh V KuchipudiHuck Institutes of the Life Sciences, Penn State University, University Park, PA 16802, USA.ORCID 0000-0002-8640-254X
Scott E LindnerHuck Institutes of the Life Sciences, Penn State University, University Park, PA 16802, USA.
Leslie J ParentDepartment of Microbiology & Immunology, Penn State College of Medicine, Hershey, PA 17033, USA.ORCID 0000-0002-0431-9049
John M FlanaganHuck Institutes of the Life Sciences, Penn State University, University Park, PA 16802, USA.
Nicholas J BuchkovichDepartment of Microbiology & Immunology, Penn State College of Medicine, Hershey, PA 17033, USA.
Pennsylvania State University · USPenn State Milton S. Hershey Medical Center · US

Funding

Pennsylvania Department of Community and Economic Development CV19-VTT
6 · The paper itself

Abstract

Antibodies targeting the spike (S) and nucleocapsid (N) proteins of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are essential tools. In addition to important roles in the treatment and diagnosis of infection, the availability of high-quality specific antibodies for the S and N proteins is essential to facilitate basic research of virus replication and in the characterization of mutations responsible for variants of concern. We have developed panels of mouse and rabbit monoclonal antibodies (mAbs) to the SARS-CoV-2 spike receptor-binding domain (S-RBD) and N protein for functional and antigenic analyses. The mAbs to the S-RBD were tested for neutralization of native SARS-CoV-2, with several exhibiting neutralizing activity. The panels of mAbs to the N protein were assessed for cross-reactivity with the SARS-CoV and Middle East respiratory syndrome (MERS)-CoV N proteins and could be subdivided into sets that showed unique specificity for SARS-CoV-2 N protein, cross-reactivity between SARS-CoV-2 and SARS-CoV N proteins only, or cross-reactivity to all three coronavirus N proteins tested. Partial mapping of N-reactive mAbs were conducted using truncated fragments of the SARS-CoV-2 N protein and revealed near complete coverage of the N protein. Collectively, these sets of mouse and rabbit monoclonal antibodies can be used to examine structure/function studies for N proteins and to define the surface location of virus neutralizing epitopes on the RBD of the S protein.

Indexed as

AnimalsAntibodies, MonoclonalAntibodies, NeutralizingAntibodies, ViralBetacoronavirusBinding SitesCoronavirus Nucleocapsid ProteinsCOVID-19Cross ReactionsEpitopesHumansMiceNeutralization TestsPhosphoproteinsProtein BindingRabbitsAntibodies, MonoclonalAntibodies, NeutralizingAntibodies, ViralCoronavirus Nucleocapsid ProteinsEpitopesnucleocapsid phosphoprotein, SARS-CoV-2PhosphoproteinsSpike Glycoprotein, Coronavirusspike protein, SARS-CoV-2Viral Envelope ProteinsantibodiescoronavirusCOVID-19nucleocapsid proteinSARS-CoV-2spike protein

Identifiers

PMID34696329
PMCPMC8537396
OpenAlexW3199510249

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.