ArticleBiophysical journal2021
Biochemical and rheological analysis of human colonic culture mucus reveals similarity to gut mucus.
Article in Biophysical journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Strategies for modelling the intestinal mucus interface: Focus on drug transport and host-microbiota interactions.International journal of pharmaceutics: X · 2026Review
- Detergent-Containing Toothpaste Decreases Esophageal Mucosal Impedance and Alters Salivary Properties in Humans.Allergy · 2026Article
- Coordinated regulation of colonic fluid and mucus secretion.Frontiers in pharmacology · 2026Review
- Development of a Caco-2-based intestinal mucosal model to study intestinal barrier properties and bacteria-mucus interactions.Gut microbes · 2025Article
- Synthetic Colonic Mucus Enables the Development of Modular Microbiome Organoids.Advanced functional materials · 2024Article
- A Microphysiological System with an Anaerobic Air-Liquid Interface and Functional Mucus Layer for Coculture of Intestinal Bacteria and Primary Human Colonic Epithelium.Advanced materials interfaces · 2024Article
- Mucus-coated, magnetically-propelled fecal surrogate to mimic fecal shear forces on colonic epithelium.Biomaterials · 2024Article
- Creation of a spatially complex mucus bilayer on an in vitro colon model.Scientific reports · 2024Article
- Article
- Mucus polymer concentration andmBio · 2024Article
- Native gastrointestinal mucus: Critical features and techniques for studying interactions with drugs, drug carriers, and bacteria.Advanced drug delivery reviews · 2023Review
- Gels That Serve as Mucus Simulants: A Review.Gels (Basel, Switzerland) · 2023Review
- Generating anCellular and molecular bioengineering · 2022Article
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Authors and funding
7 authors.
Funding
Abstract
The goal of this project was to validate the functional relevance and utility of mucus produced by an in vitro intestinal cell culture model. This is facilitated by the need to physiologically replicate both healthy and abnormal mucus conditions from native intestinal tissue, where mucus properties have been connected to intestinal disease models. Mucus harvested from colonic cell cultures derived from healthy donors was compared to mucus collected from surgically resected, noninflamed transverse colon tissue. The rheological and biochemical properties of these mucus samples were compared using oscillational rheometry, particle-tracking microrheology, multiangle laser light scattering, refractometry, and immunohistochemical imaging. An air-liquid interface culture of primary human colonic epithelial cells generated a continuous monolayer with an attached mucus layer that displayed increasing weight percent (wt%) of solids over 1 week (1.3 ± 0.5% at 2 days vs. 2.4 ± 0.3% at 7 days). The full range of mucus concentrations (0.9-3.3%) observed during culture was comparable to that displayed by ex vivo mucus (1.3-1.9%). Bulk rheological measurements displayed similar wt%-based complex viscosities between in vitro and ex vivo mucus, with the complex viscosity of both systems increasing with wt% of solids. Particle-tracking microrheology showed higher complex viscosities for ex vivo mucus samples than in vitro mucus which was explained by a greater fraction of water present in in vitro mucus than ex vivo, i.e., in vitro mucus is more heterogeneous than ex vivo. Refractometry, multiangle laser light scattering, and immunostaining showed increased mucus complex size in ex vivo mucus compared with in vitro mucus, which may have been due to the admixture of mucus and cellular debris during ex vivo mucus collection. The air-liquid interface culture system produced intestinal mucus with similar composition and rheology to native human gut mucus, providing a platform to analyze pathological differences in intestinal mucus.
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Registered trials
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