Evidence map›Paper›PMID 34695122›Full record

ArticlePloS one2021

Validation study of Boil & Spin Malachite Green Loop Mediated Isothermal Amplification (B&S MG-LAMP) versus microscopy for malaria detection in the Peruvian Amazon.

Keare A Barazorda, Carola J Salas, Greys Braga, Leonila Ricopa, Julia S Ampuero, Crystyan Siles, Juan F Sanchez, Silvia Montano, Stephen E Lizewski, Christie A Joya and 2 more

Open access · goldAbstract readValidation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.2field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Recombinase-Aided Loop-Mediated Isothermal Amplification on Human Plasmodium knowlesi.The American journal of tropical medicine and hygiene · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 1 institution in 1 country.

Keare A BarazordaVysnova Partners Inc, Lima, Peru.
Carola J SalasDepartment of Parasitology, U.S. Naval Medical Research Unit N°6 (NAMRU-6), Lima, Peru.
Greys BragaDepartment of Parasitology, U.S. Naval Medical Research Unit N°6 (NAMRU-6), Lima, Peru.
Leonila RicopaDepartment of Parasitology, U.S. Naval Medical Research Unit N°6 (NAMRU-6), Lima, Peru.
Julia S AmpueroDepartment of Virology and Emerging infections, U.S. Naval Medical Research Unit N°6 (NAMRU-6), Lima, Peru.
Crystyan SilesDepartment of Virology and Emerging infections, U.S. Naval Medical Research Unit N°6 (NAMRU-6), Lima, Peru.
Juan F SanchezDepartment of Parasitology, U.S. Naval Medical Research Unit N°6 (NAMRU-6), Lima, Peru.
Silvia MontanoDepartment of Parasitology, U.S. Naval Medical Research Unit N°6 (NAMRU-6), Lima, Peru.
Stephen E LizewskiDepartment of Parasitology, U.S. Naval Medical Research Unit N°6 (NAMRU-6), Lima, Peru.
Christie A JoyaDepartment of Parasitology, U.S. Naval Medical Research Unit N°6 (NAMRU-6), Lima, Peru.
Danett K BishopDepartment of Parasitology, U.S. Naval Medical Research Unit N°6 (NAMRU-6), Lima, Peru.
Hugo O ValdiviaDepartment of Parasitology, U.S. Naval Medical Research Unit N°6 (NAMRU-6), Lima, Peru.ORCID 0000-0003-2159-5667
United States Naval Medical Research Unit SOUTH · PE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Malaria elimination efforts in Peru have dramatically reduced the incidence of cases in the Amazon Basin. To achieve the elimination, the detection of asymptomatic and submicroscopic carriers becomes a priority. Therefore, efforts should focus on tests sensitive enough to detect low-density parasitemia, deployable to resource-limited areas and affordable for large screening purposes. In this study, we assessed the performance of the Malachite-Green LAMP (MG-LAMP) using heat-treated DNA extraction (Boil & Spin; B&S MG-LAMP) on 283 whole blood samples collected from 9 different sites in Loreto, Peru and compared its performance to expert and field microscopy. A real-time PCR assay was used to quantify the parasite density. In addition, we explored a modified version of the B&S MG-LAMP for detection of submicroscopic infection in 500 samples and compared the turnaround time and cost of the MG-LAMP with microscopy. Compared to expert microscopy, the genus B&S MG-LAMP had a sensitivity of 99.4% (95%CI: 96.9%- 100%) and specificity of 97.1% (95%CI: 91.9%- 99.4%). The P. vivax specific B&S MG-LAMP had a sensitivity of 99.4% (96.6%- 100%) and specificity of 99.2% (95.5%- 100%) and the P. falciparum assay had a sensitivity of 100% (95%CI: 78.2%- 100%) and specificity of 99.3% (95%CI: 97.3%- 99.8%). The modified genus B&S MG-LAMP assay detected eight submicroscopic malaria cases (1.6%) which the species-specific assays did not identify. The turnaround time of B&S MG-LAMP was faster than expert microscopy with as many as 60 samples being processed per day by field technicians with limited training and utilizing a simple heat-block. The modified B&S MG-LAMP offers a simple and sensitive molecular test of choice for the detection of submicroscopic infections that can be used for mass screening in resources limited facilities in endemic settings nearing elimination and where a deployable test is required.

Indexed as

HumansMalaria, FalciparumMicroscopyMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesPeruPlasmodium falciparumRosaniline Dyesmalachite greenRosaniline Dyes

Identifiers

PMID34695122
PMCPMC8544869
OpenAlexW3209878737

What OpenQuestion holds

Textmetadata
LicenceCC0
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.