Evidence map›Paper›PMID 34694622›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Transcriptome-Wide Identification of 2'-O-Methylation Sites with RibOxi-Seq.

Yinzhou Zhu, Christopher L Holley, Gordon G Carmichael

Open access · greenAbstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.6field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 9 citations in OpenAlex.

  1. SnoRNA Expression and RNA 2'-O-Methylation inbioRxiv : the preprint server for biology · 2026
    Article
  2. Article
  3. Genome-wide profiling of RNA 2'-bioRxiv : the preprint server for biology · 2025
    Article
  4. Article
  5. Article
  6. Article
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Yinzhou ZhuDepartment of Medicine (Cardiology Division), Duke University School of Medicine, Durham, NC, USA.
Christopher L HolleyDepartment of Medicine (Cardiology Division), Duke University School of Medicine, Durham, NC, USA.
Gordon G CarmichaelDepartment of Genetics and Genome Sciences, UCONN Health, Farmington, CT, USA. carmichael@uchc.edu.
Duke University · USUConn Health · US

Funding

Molecular underpinnings of Prader-Willi syndromeR01HD099975 · NICHD · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI CARMICHAEL, GORDON G, COTNEY, JUSTIN LEE · 2019 to 2023
$2.9M
The Role of RNA Modifications in Heart FailureR01HL146381 · NHLBI · DUKE UNIVERSITY · PI HOLLEY, CHRISTOPHER LEE · 2019 to 2023
$1.8M
SnoRNA-guided modifications of mRNAR01GM135383 · NIGMS · DUKE UNIVERSITY · PI HOLLEY, CHRISTOPHER LEE · 2020 to 2023
$1.3M
NHLBI NIH HHS R01 HL146381NICHD NIH HHS R01 HD099975NIGMS NIH HHS R01 GM135383
6 · The paper itself

Abstract

The ability to detect 2'-O-methylation sites (Nm) in high-throughput fashion is important, as increasing evidence points to a more diverse landscape for this RNA modification as well as the possibility of yet unidentified functions. Here we describe an optimized version of RibOxi-seq, which is built upon the original published method, that not only accurately profiles ribosomal RNA (rRNA) Nm sites with minimal RNA input but is also robust enough to identify mRNA intronic and exonic sites.

Indexed as

TranscriptomeBase SequenceMethylationRNARNA, RibosomalRNARNA, Ribosomal2′-O-methylationmRNA modificationsNmRibose methylationRNA modificationsrRNA modifications

Identifiers

PMID34694622
PMCPMC9165532
OpenAlexW3210324437

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.