Evidence map›Paper›PMID 34694614›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Single-Molecule RNA Imaging Using Mango II Arrays.

Adam D Cawte, Haruki Iino, Peter J Unrau, David S Rueda

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Translation Dynamics of Single mRNAs in Live Cells.Annual review of biophysics · 2024
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Adam D CawteSingle Molecule Imaging Group, MRC London Institute of Medical Sciences, London, UK.
Haruki IinoSingle Molecule Imaging Group, MRC London Institute of Medical Sciences, London, UK.
Peter J UnrauDepartment of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC, Canada. punrau@sfu.ca.
David S RuedaSingle Molecule Imaging Group, MRC London Institute of Medical Sciences, London, UK. david.rueda@imperial.ac.uk.

Funding

Medical Research Council MC-A658-5TY10Medical Research Council MC_UP_1102/5Wellcome Trust P67153
6 · The paper itself

Abstract

In recent years, fluorogenic RNA aptamers, such as Spinach, Broccoli, Corn, Mango, Coral, and Pepper have gathered traction as an efficient alternative labeling strategy for background-free imaging of cellular RNAs. However, their application has been somewhat limited by relatively inefficient folding and fluorescent stability. With the recent advent of novel RNA-Mango variants which are improved in both fluorescence intensity and folding stability in tandem arrays, it is now possible to image RNAs with single-molecule sensitivity. Here we discuss the protocol for imaging Mango II tagged RNAs in both fixed and live cells.

Indexed as

MangiferaAptamers, NucleotideFluorescent DyesRNASpinacia oleraceaAptamers, NucleotideFluorescent DyesRNAFluorogenic RNA AptamerLive cell imagingRNA dynamicsRNA-MangoSingle-Molecule MicroscopySuperresolution microscopy

Identifiers

PMID34694614

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.