Evidence map›Paper›PMID 34693626›Full record

ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2022

OMIP-080: 29-Color flow cytometry panel for comprehensive evaluation of NK and T cells reconstitution after hematopoietic stem cells transplantation.

Sarka Vanikova, Abhishek Koladiya, Jan Musil

Registry-linked trialAbstract read
In one paragraph

Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT06281262 (The Role of Selected Immunological Indicators and Microbiota in Patients Experiencing Premature Birth and Preeclampsia), which is not on this map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT06281262 unknown statusnot on this mapstarted 2023, after this paper: background citation

The Role of Selected Immunological Indicators and Microbiota in Patients Experiencing Premature Birth and Preeclampsia

TypeobservationalSponsorGeneral University Hospital, PragueRan2023 to 2025Enrolled100ConditionsPreterm Birth, PreeclampsiaArmsPeripheral blood collection, Swab sample collection
3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. OMIP-099: 31-color spectral flow cytometry panel to investigate the steady-state phenotype of human T cells.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2024
    Article
  9. Article
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Sarka VanikovaDepartment of Immunomonitoring and Flow Cytometry, Institute of Hematology and Blood Transfusion, Prague, Czech Republic.
Abhishek KoladiyaDepartment of Immunomonitoring and Flow Cytometry, Institute of Hematology and Blood Transfusion, Prague, Czech Republic.
Jan MusilDepartment of Immunomonitoring and Flow Cytometry, Institute of Hematology and Blood Transfusion, Prague, Czech Republic.ORCID 0000-0002-9378-5956

Funding

European Regional Development Fund and the state budget of the Czech Republic, project AIIHHP CZ.02.1.01/0.0/0.0/16_025/0007428 OP RDE MEYSH CZ - DRO (Institute of Hematology and Blood Tranfusion - IHBT) IN 00023736Ministerstvo Zdravotnictví Ceské Republiky
6 · The paper itself

Abstract

This 29-color panel was developed and optimized for the monitoring of NK cell and T cell reconstitution in peripheral blood of patients after HSCT. We considered major post-HSCT complications during the design, such as relapses, viral infections, and GvHD and identification of lymphocyte populations relevant to their resolution. The panel includes markers for all major NK cell and T cell subsets and analysis of their development and qualitative properties. In the NK cell compartment, we focus mainly on CD57 + NKG2C+ cells and the expression of activating (NKG2D, DNAM-1) and inhibitory receptors (NKG2A, TIGIT). Another priority is the characterization of T cell reconstitution; therefore, we included detection of CD4+ RTEs based on CD45RA, CD62L, CD95, and CD31 as a marker of thymus function. Besides that, we also analyze the emergence and properties of major T cell populations with a particular interest in CD8, Th1, ThCTL, and Treg subsets. Overall, the panel allows for comprehensive analysis of the reconstituting immune system and identification of potential markers of immune cell dysfunction.

Indexed as

Hematopoietic Stem Cell TransplantationFlow CytometryHematopoietic Stem CellsHumansLymphocyte CountT-Lymphocyte Subsets

Identifiers

PMID34693626
PMCPMC9298022

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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.