ArticleBiomedical optics express2021
Expansion tomography for large volume tissue imaging with nanoscale resolution.
Article in Biomedical optics express, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Whole-organ single-cell mapping defines growth dynamics and clonal organization in developing and adult zebrafish livers.PLoS biology · 2026Article
- Maximising imaging volumes of expanded tissues for inverted fluorescence microscopy.Biomedical optics express · 2026Article
- Mesoscale volumetric fluorescence imaging at nanoscale resolution by photochemical sectioning.Science (New York, N.Y.) · 2025Article
- Article
- Rapid lightsheet fluorescence imaging of whole Drosophila brains at nanoscale resolution by potassium acrylate-based expansion microscopy.Nature communications · 2024Article
- Expanding boundaries - a cell biologist's guide to expansion microscopy.Journal of cell science · 2024Review
- GelMap: intrinsic calibration and deformation mapping for expansion microscopy.Nature methods · 2023Article
- Visualizing cellular and tissue ultrastructure using Ten-fold Robust Expansion Microscopy (TREx).eLife · 2022Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Expansion microscopy enables conventional diffraction limit microscopy to achieve super-resolution imaging. However, the enlarged tissue lacks an objective lens with sufficient working distance that can image tissues with whole-brain-scale coverage. Here, we present expansion tomography (ExT) to solve this problem. We have established a modified super-absorbent hydrogel (ExT gel) that possesses high mechanical strength and enables serial sectioning. ExT gel enables tissue and cell imaging and is compatible with various fluorescent labeling strategies. Combining with the high-throughput light-sheet tomography (HLTP) system, we have shown the capability of large volume imaging with nanoscale resolution of mouse brain intact neuronal circuits. The ExT method would allow image samples to support super-resolution imaging of intact tissues with virtually unlimited axial extensions.
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Registered trials
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