ArticleBiomedical optics express2021
Dec-DISCO: decolorization DISCO clearing for seeing through the biological architectures of heme-rich organs.
Article in Biomedical optics express, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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Who cites it
5 citing papers in PubMed.
- Optimizing tissue clearing methods for improved imaging of whole-mount retinas and optic nerves.Journal of neuroscience methods · 2025Article
- Advances in tissue optical clearing for 3D imaging in large animal.Frontiers of optoelectronics · 2025Review
- Strongly absorbing molecules make tissue transparent: a new insight for understanding tissue optical clearing.Light, science & applications · 2025Article
- FLUID: a fluorescence-friendly lipid-compatible ultrafast clearing method.Biomedical optics express · 2024Article
- Repeated epidural delivery of Shinbaro2: effects on neural recovery, inflammation, and pain modulation in a rat model of lumbar spinal stenosis.Frontiers in pharmacology · 2024Article
Corrections and comments
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Authors and funding
8 authors.
Funding
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Abstract
The tissue optical clearing technique plays an important role in three-dimensional (3D) visualization of large tissues. As a typical solvent-based clearing method, 3DISCO can achieve the highest level of tissue transparency with favorable clearing speed. However, 3DISCO cannot deal with the residual blood within tissues, leading to tissue brownness or redness after clearing, thus greatly influencing the tissue transparency and image quality due to the strong absorption of residual blood. To address this problem, we proposed an optimized clearing method by introducing CUBIC-L solution combined with 3DISCO for effective decolorization, termed Dec-DISCO (Decolorization DISCO). Dec-DISCO achieves better transparency than 3DISCO for various heme-rich tissues and performs enhanced fluorescence preservation capability. Dec-DISCO allows high-quality 3D imaging of fluorescently labeled heme-rich organs, as well as pathological tissue with severe hemorrhage. Dec-DISCO is expected to provide a powerful tool for histological analysis of kinds of heme-rich tissues in various medical conditions.
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Registered trials
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