ArticleThe ISME journal2022
Activity-based, genome-resolved metagenomics uncovers key populations and pathways involved in subsurface conversions of coal to methane.
Article in The ISME journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 45 citations in OpenAlex.
- Molecular bioindicators for assessing environmental impacts of emerging contaminants.Fundamental research · 2026Review
- Divergent mechanisms of active antibiotic resistance gene enrichment in soil driven by pesticide diversity.Nature communications · 2026Article
- Article
- Graphitized Biochar Derived from Agricultural Wastes Enhances Methanogenesis via Conductivity-Driven Direct Interspecies Electron Transfer.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- An innovative strategy for overcoming ultra-high ammonia nitrogen inhibition on anaerobic methanogenesis via stepwise domestication.Microbiome · 2025Article
- Recent progress on the biological degradation and solubilization of coal.Biodegradation · 2025Review
- Seasonal Variations of Community Structure and Functional Genes ofMicroorganisms · 2025Article
- Anthropogenic impacts on the terrestrial subsurface biosphere.Nature reviews. Microbiology · 2025Review
- Comparative genomic analysis and characterization of novel high-quality draft genomes from the coal metagenome.World journal of microbiology & biotechnology · 2024Article
- Metatranscriptomics-guided genome-scale metabolic reconstruction reveals the carbon flux and trophic interaction in methanogenic communities.Microbiome · 2024Article
- New insights into the coal-associated methane architect: the ancient archaebacteria.Archives of microbiology · 2024Review
- Article
- Selective trace elements significantly enhanced methane production in coal bed methane systems by stimulating microbial activity.Microbiology spectrum · 2024Article
- Metagenomic data from surface seawater of the east coast of South Korea.Scientific data · 2023Article
- Using click chemistry to study microbial ecology and evolution.ISME communications · 2023Review
- Natural and anthropogenic carbon input affect microbial activity in salt marsh sediment.Frontiers in microbiology · 2023Article
- Algal amendment enhances biogenic methane production from coals of different thermal maturity.Frontiers in microbiology · 2023Article
- Expression of divergent methyl/alkyl coenzyme M reductases from uncultured archaea.Communications biology · 2022Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microbial metabolisms and interactions that facilitate subsurface conversions of recalcitrant carbon to methane are poorly understood. We deployed an in situ enrichment device in a subsurface coal seam in the Powder River Basin (PRB), USA, and used BONCAT-FACS-Metagenomics to identify translationally active populations involved in methane generation from a variety of coal-derived aromatic hydrocarbons. From the active fraction, high-quality metagenome-assembled genomes (MAGs) were recovered for the acetoclastic methanogen, Methanothrix paradoxum, and a novel member of the Chlorobi with the potential to generate acetate via the Pta-Ack pathway. Members of the Bacteroides and Geobacter also encoded Pta-Ack and together, all four populations had the putative ability to degrade ethylbenzene, phenylphosphate, phenylethanol, toluene, xylene, and phenol. Metabolic reconstructions, gene analyses, and environmental parameters also indicated that redox fluctuations likely promote facultative energy metabolisms in the coal seam. The active "Chlorobi PRB" MAG encoded enzymes for fermentation, nitrate reduction, and multiple oxygenases with varying binding affinities for oxygen. "M. paradoxum PRB" encoded an extradiol dioxygenase for aerobic phenylacetate degradation, which was also present in previously published Methanothrix genomes. These observations outline underlying processes for bio-methane from subbituminous coal by translationally active populations and demonstrate activity-based metagenomics as a powerful strategy in next generation physiology to understand ecologically relevant microbial populations.
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