ArticleThe Science of the total environment2022
High throughput sequencing based direct detection of SARS-CoV-2 fragments in wastewater of Pune, West India.
Article in The Science of the total environment, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Article
- Article
- Unsupervised detection of novel SARS-CoV-2 mutations and lineages in wastewater samples using long-read sequencing.BMC genomics · 2025Article
- Respiratory virus infection dynamics and genomic surveillance to detect seasonal influenza subtypes in wastewater: A longitudinal study in Bengaluru, India.PLOS global public health · 2025Article
- Wastewater-based genomic surveillance of SARS-CoV-2 in vulnerable communities in Mumbai.The Indian journal of medical research · 2024Article
- Tracking epidemic viruses in wastewaters.Microbial biotechnology · 2024Review
- Pathogen genomic surveillance status among lower resource settings in Asia.Nature microbiology · 2024Article
- Towards geospatially-resolved public-health surveillance via wastewater sequencing.Nature communications · 2024Article
- Surveillance of influenza A and B viruses from community and hospital wastewater treatment plants.Environmental microbiology reports · 2024Article
- Tracking SARS-CoV-2 variants of concern in wastewater: an assessment of nine computational tools using simulated genomic data.Microbial genomics · 2024Article
- Article
- Nanopore sequencing technology and its applications.MedComm · 2023Review
- SARS-CoV-2 infection dynamics and genomic surveillance to detect variants in wastewater - a longitudinal study in Bengaluru, India.The Lancet regional health. Southeast Asia · 2023Article
- Toxicity assessment of SARS-CoV-2-derived peptides in combination with a mix of pollutants on zebrafish adults: A perspective study of behavioral, biometric, mutagenic, and biochemical toxicity.The Science of the total environment · 2023Article
- Viral Metagenomics as a Tool to Track Sources of Fecal Contamination: A One Health Approach.Viruses · 2023Review
- Identification of Genomic Variants of SARS-CoV-2 Using Nanopore Sequencing.Medicina (Kaunas, Lithuania) · 2022Article
- Short-term exposure of the mayfly larvae (Cloeon dipterum, Ephemeroptera: Baetidae) to SARS-CoV-2-derived peptides and other emerging pollutants: A new threat for the aquatic environments.The Science of the total environment · 2022Article
- Monitoring the evolution of SARS-CoV-2 on a Spanish university campus through wastewater analysis: A pilot project for the reopening strategy.The Science of the total environment · 2022Article
- Wastewater-based epidemiology in countries with poor wastewater treatment - Epidemiological indicator function of SARS-CoV-2 RNA in surface waters.The Science of the total environment · 2022Article
- Genetic sequencing detected the SARS-CoV-2 delta variant in wastewater a month prior to the first COVID-19 case in Ahmedabad (India).Environmental pollution (Barking, Essex : 1987) · 2022Article
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9 authors.
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Abstract
Given a large number of SARS-CoV-2 infected individuals, clinical detection has proved challenging. The wastewater-based epidemiological paradigm would cover the clinically escaped asymptomatic individuals owing to the faecal shedding of the virus. We hypothesised using wastewater as a valuable resource for analysing SARS-CoV-2 mutations circulating in the wastewater of Pune region (Maharashtra; India), one of the most affected during the covid-19 pandemic. We conducted study in open wastewater drains from December 2020-March 2021 to assess the presence of SARS-CoV-2 nucleic acid and further detect mutations using ARTIC protocol of MinION sequencing. The analysis revealed 108 mutations across six samples categorised into 39 types of mutations. We report the occurrence of mutations associated with Delta variant lineage in March-2021 samples, simultaneously also reported as a Variant of Concern (VoC) responsible for the rapid increase in infections. The study also revealed four mutations; S:N801, S:C480R, NSP14:C279F and NSP3:L550del not currently reported from wastewater or clinical data in India but reported worldwide. Further, a novel mutation NSP13:G206F mapping to NSP13 region was observed from wastewater. Notably, S:P1140del mutation was detected in December 2020 samples while it was reported in February 2021 from clinical data, indicating the instrumentality of wastewater data in early detection. This is the first study in India to demonstrate utility of sequencing in wastewater-based epidemiology to identify mutations associated with SARS-CoV-2 virus fragments from wastewater as an early warning indicator system.
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