ArticleMethods in molecular biology (Clifton, N.J.)2022
Pure Culture and DNA Sequence-Based Identification of Fusarium from Symptomatic Plants and Diverse Substrates.
Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed, 15 citations in OpenAlex.
- Integrated Assessment of Fungi Contamination and Mycotoxins Levels Across the Rice Processing Chain.Toxins · 2025Article
- Fusarium spp. associated with Chenopodium quinoa crops in Colombia.Scientific reports · 2022Article
- Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fusarium ranks as the most important group of plant pathogens, responsible for a wide range of economically destructive diseases, including vascular wilts and root, crown, and stem rots. In addition, head blight and ear rot diseases are associated with the accumulation of mycotoxins in cereals. With over 300 phylogenetically distinct species, and a dearth of phenotypical characteristics, DNA sequence data in most instances is the only reliable means for obtaining an accurate species identification. Here we describe how to obtain single-spored pure cultures from symptomatic host tissue and a molecular identification by querying publicly accessible DNA sequence databases using a portion of translation elongation factor 1-α (TEF1), the largest subunit of RNA polymerase (RPB1), and/or the second largest subunit of RNA polymerase (RPB2).
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