Evidence map›Paper›PMID 34686698›Full record

ArticleScientific reports2021

Wnt6 plays a complex role in maintaining human limbal stem/progenitor cells.

Clémence Bonnet, Denise Oh, Hua Mei, Sarah Robertson, Derek Chang, Jean-Louis Bourges, Francine Behar-Cohen, Jie J Zheng, Sophie X Deng

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 18 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Emerging roles and biomarker potential of WNT6 in human cancers.Cell communication and signaling : CCS · 2024
    Review
  5. Review
  6. Article
  7. Article
  8. Article
  9. Review
  10. Regulation of Limbal Epithelial Stem Cells: Importance of the Niche.International journal of molecular sciences · 2021
    Review
  11. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Clémence Bonnet *Stein Eye Institute, University of California, Los Angeles, 200 Stein Plaza, Los Angeles, CA, 90095, USA.
Denise Oh *Stein Eye Institute, University of California, Los Angeles, 200 Stein Plaza, Los Angeles, CA, 90095, USA.
Hua MeiDepartment of Ophthalmology, University of North Carolina, Chapel Hill, NC, USA.
Sarah RobertsonStein Eye Institute, University of California, Los Angeles, 200 Stein Plaza, Los Angeles, CA, 90095, USA.
Derek ChangStein Eye Institute, University of California, Los Angeles, 200 Stein Plaza, Los Angeles, CA, 90095, USA.
Jean-Louis BourgesINSERM, UMRS1138, Team 17, From Physiopathology of Ocular Diseases to Clinical Development, Paris University, Centre de Recherche des Cordeliers, and Ophthalmology Department, Cochin Hospital, AP-HP, 75014, Paris, France.
Francine Behar-CohenINSERM, UMRS1138, Team 17, From Physiopathology of Ocular Diseases to Clinical Development, Paris University, Centre de Recherche des Cordeliers, and Ophthalmology Department, Cochin Hospital, AP-HP, 75014, Paris, France.
Jie J ZhengStein Eye Institute, University of California, Los Angeles, 200 Stein Plaza, Los Angeles, CA, 90095, USA.
Sophie X DengStein Eye Institute, University of California, Los Angeles, 200 Stein Plaza, Los Angeles, CA, 90095, USA. deng@jsei.ucla.edu.
University of California, Los Angeles · USCentre de Recherche des Cordeliers · FRUniversity of North Carolina at Chapel Hill · US

Funding

Vision Research Core at UCLAP30EY000331 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Xian-Jie Yang · 1985 to 2026
$16.8M
Ex Vivo Expansion of Human Limbal Stem Cells for TransplantationR01EY021797 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Sophie Deng · 2012 to 2026
$5.1M
Development of small-molecule Wnt mimetics for corneal epithelial cell regenerationR01EY028557 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI DENG, SOPHIE, ZHENG, JIE J. · 2018 to 2021
$2.0M
NEI NIH HHS P30 EY000331NEI NIH HHS R01 EY021797NEI NIH HHS R01 EY028557
6 · The paper itself

Abstract

The corneal epithelium is consistently regenerated by limbal stem/progenitor cells (LSCs), a very small population of adult stem cells residing in the limbus. Several Wnt ligands, including Wnt6, are preferentially expressed in the limbus. To investigate the role of Wnt6 in regulating proliferation and maintenance of human LSCs in an in vitro LSC expansion setting, we generated NIH-3T3 feeder cells to overexpress different levels of Wnt6. Characterization of LSCs cultured on Wnt6 expressing 3T3 cells showed that high level of Wnt6 increased proliferation of LSCs. Medium and high levels of Wnt6 also increased the percentage of small cells (diameter ≤ 12 µm), a feature of the stem cell population. Additionally, the percentage of cells expressing the differentiation marker K12 was significantly reduced in the presence of medium and high Wnt6 levels. Although Wnt6 is mostly known as a canonical Wnt ligand, our data showed that canonical and non-canonical Wnt signaling pathways were activated in the Wnt6-supplemented LSC cultures, a finding suggesting that interrelationships between both pathways are required for LSC regulation.

Indexed as

AdultAgedAnimalsAntigens, DifferentiationCell Culture TechniquesCell DifferentiationCell LineCell ProliferationEpithelial CellsEpithelium, CornealHumansLimbus CorneaeMiceMiddle AgedNIH 3T3 CellsProto-Oncogene ProteinsAntigens, DifferentiationProto-Oncogene ProteinsWNT6 protein, humanWnt6 protein, mouseWnt Proteins

Identifiers

PMID34686698
PMCPMC8536737
OpenAlexW3188428908

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.