Evidence map›Paper›PMID 34686208›Full record

ArticleBMC veterinary research2021

Characterisation and preliminary functional analysis of N-acetyltransferase 13 from Schistosoma japonicum.

Yalan Tang, Kerou Zhou, Qingqing Guo, Cheng Chen, Jing Jia, Qinghong Guo, Ke Lu, Hao Li, Zhiqiang Fu, Jinming Liu and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 90% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 1 country.

Yalan Tang *National Reference Laboratory for Animal Schistosomiasis, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No.518 Ziyue Road, Minhang District, Shanghai, 200241, People's Republic of China.
Kerou Zhou *National Reference Laboratory for Animal Schistosomiasis, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No.518 Ziyue Road, Minhang District, Shanghai, 200241, People's Republic of China.
Qingqing GuoNational Reference Laboratory for Animal Schistosomiasis, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No.518 Ziyue Road, Minhang District, Shanghai, 200241, People's Republic of China.
Cheng ChenNational Reference Laboratory for Animal Schistosomiasis, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No.518 Ziyue Road, Minhang District, Shanghai, 200241, People's Republic of China.
Jing JiaNational Reference Laboratory for Animal Schistosomiasis, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No.518 Ziyue Road, Minhang District, Shanghai, 200241, People's Republic of China.
Qinghong GuoNational Reference Laboratory for Animal Schistosomiasis, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No.518 Ziyue Road, Minhang District, Shanghai, 200241, People's Republic of China.
Ke LuNational Reference Laboratory for Animal Schistosomiasis, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No.518 Ziyue Road, Minhang District, Shanghai, 200241, People's Republic of China.
Hao LiNational Reference Laboratory for Animal Schistosomiasis, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No.518 Ziyue Road, Minhang District, Shanghai, 200241, People's Republic of China.
Zhiqiang FuNational Reference Laboratory for Animal Schistosomiasis, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No.518 Ziyue Road, Minhang District, Shanghai, 200241, People's Republic of China.
Jinming LiuNational Reference Laboratory for Animal Schistosomiasis, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No.518 Ziyue Road, Minhang District, Shanghai, 200241, People's Republic of China.
Jiaojiao LinNational Reference Laboratory for Animal Schistosomiasis, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No.518 Ziyue Road, Minhang District, Shanghai, 200241, People's Republic of China.
Xingang YuCollege of Life Science and Engineering, Foshan University, Foshan, 528231, People's Republic of China. 898461789@qq.com.
Yang HongNational Reference Laboratory for Animal Schistosomiasis, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No.518 Ziyue Road, Minhang District, Shanghai, 200241, People's Republic of China. hongyang_7@126.com.
Chinese Academy of Agricultural Sciences · CNShanghai Veterinary Research Institute · CNFoshan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundN-acetyltransferase 13 (NAT13) is a probable catalytic component of the ARD1A-NARG1 complex possessing alpha (N-terminal) acetyltransferase activity.

resultsIn this study, a full-length complementary DNA (cDNA) encoding Schistosoma japonicum NAT13 (SjNAT13) was isolated from schistosome cDNAs. The 621 bp open reading frame of SjNAT13 encodes a polypeptide of 206 amino acids. Real-time PCR analysis revealed SjNAT13 expression in all tested developmental stages. Transcript levels were highest in cercariae and 21-day-old worms, and higher in male adult worms than female adult worms. The rSjNAT13 protein induced high levels of anti-rSjNAT13 IgG antibodies. In two independent immunoprotection trials, rSjNAT13 induced 24.23% and 24.47% reductions in the numbers of eggs in liver. RNA interference (RNAi) results showed that small interfering RNA (siRNA) Sj-514 significantly reduced SjNAT13 transcript levels in worms and decreased egg production in vitro.

conclusionsThus, rSjNAT13 might play an important role in the development and reproduction of schistosomes.

Indexed as

AcetyltransferasesAnimalsCloning, MolecularDNA, ComplementaryFemaleGene Expression Regulation, EnzymologicGene SilencingHelminth ProteinsMaleMiceMice, Inbred BALB CRandom AllocationRNA, MessengerSchistosoma japonicumSchistosomiasis japonicaVaccinesAcetyltransferasesDNA, ComplementaryHelminth ProteinsRNA, MessengerVaccinesDevelopmentN-acetyltransferaseReproductionRNAiSchistosoma japonicumSchistosomeSjNAT13

Identifiers

PMID34686208
PMCPMC8540080
OpenAlexW3205183540

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.