ArticlePlants (Basel, Switzerland)2021
Sensitive and Rapid Detection of Citrus Scab Using an RPA-CRISPR/Cas12a System Combined with a Lateral Flow Assay.
Article in Plants (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 42 citations in OpenAlex.
- Early and on-site detection of Ganoderma-induced basal stem rot in oil palm: using recombinase polymerase amplification-lateral flow assay.Folia microbiologica · 2026Article
- Research progress on nucleic acid amplification-based detection technologies for phytopathogenic fungi.Applied microbiology and biotechnology · 2026Review
- Establishment of a Rapid Detection Technique Based on RPA-LFD and RPA-CRISPR/Cas12a onMicroorganisms · 2025Article
- Single and dual RPA-CRISPR/Cas assays for point-of-need detection of Stewart's wilt pathogen (Pantoea stewartii subsp. stewartii) of corn and Maize dwarf mosaic virus.Pest management science · 2025Article
- New frontiers in CRISPR: Addressing antimicrobial resistance with Cas9, Cas12, Cas13, and Cas14.Heliyon · 2025Review
- A combined recombinase polymerase amplification CRISPR/Cas12a assay for detection of Fusarium oxysporum f. sp. cubense tropical race 4.Scientific reports · 2025Article
- RPA-assisted CRISPR-Cas12a-enabled point-of-care diagnostic platform for chili leaf curl virus with fluorescent and colorimetric readouts.Frontiers in microbiology · 2025Article
- A novel system with robust compatibility and stability for detectingMicrobiology spectrum · 2024Article
- Identification of Viruses InfectingInternational journal of molecular sciences · 2024Article
- CRISPR/Cas12a-based method coupled with isothermal amplification to identifyFrontiers in microbiology · 2024Article
- CRISPR-based nucleic acid diagnostics for pathogens.Trends in analytical chemistry : TRAC · 2023Review
- Molecular Approaches for Detection ofBiology · 2023Review
- RPA-CRISPR/Cas12a mediated isothermal amplification for visual detection ofFrontiers in cellular and infection microbiology · 2023Article
- CRISPR/Cas12a-based approaches for efficient and accurate detection ofFrontiers in cellular and infection microbiology · 2023Article
- Diagnostics of Infections Produced by the Plant Viruses TMV, TEV, and PVX with CRISPR-Cas12 and CRISPR-Cas13.ACS synthetic biology · 2022Article
- Development of a Rapid and Efficient RPA-CRISPR/Cas12a Assay forFrontiers in microbiology · 2022Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Citrus is the most extensively produced fruit tree crop in the world and is grown in over 130 countries. Fungal diseases in citrus can cause significant losses in yield and quality. An accurate diagnosis is critical for determining the best management practices and preventing future losses. In this study, a Recombinase polymerase amplification (RPA)-clustered regularly interspaced short palindromic repeats (CRISPR)/associated (Cas) system was established with the integration of a lateral flow assay (LFA) readout system for diagnosis of citrus scab. This detection can be completed within 1 h, is highly sensitive and prevents cross-reactions with other common fungal citrus diseases. Furthermore, the detection system is compatible with crude DNA extracted from infected plant tissue. This RPA-CRISPR/Cas12a-LFA system provides a sensitive, rapid, and cost-effective method with promising and significant practical value for point-of-care diagnosis of citrus scab. To our knowledge, this is the first report to establish an RPA- and CRISPR-based method with LFA for fungal diseases in plants.
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