Evidence map›Paper›PMID 34684775›Full record

ArticleMolecules (Basel, Switzerland)2021

A Neoglycoprotein-Immobilized Fluorescent Magnetic Bead Suspension Multiplex Array for Galectin-Binding Studies.

Libo Zhang, Hai Yu, Yuanyuan Bai, Bijoyananda Mishra, Xiaoxiao Yang, Jing Wang, Evan B Yu, Riyao Li, Xi Chen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecules (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Libo ZhangDepartment of Chemistry, University of California, Davis, CA 95616, USA.
Hai YuDepartment of Chemistry, University of California, Davis, CA 95616, USA.
Yuanyuan BaiDepartment of Chemistry, University of California, Davis, CA 95616, USA.
Bijoyananda MishraDepartment of Chemistry, University of California, Davis, CA 95616, USA.
Xiaoxiao YangDepartment of Chemistry, University of California, Davis, CA 95616, USA.
Jing WangDepartment of Chemistry, University of California, Davis, CA 95616, USA.
Evan B YuDepartment of Chemistry, University of California, Davis, CA 95616, USA.
Riyao LiDepartment of Chemistry, University of California, Davis, CA 95616, USA.
Xi ChenDepartment of Chemistry, University of California, Davis, CA 95616, USA.ORCID 0000-0002-3160-614X
University of California, Davis · USChinese Academy of Sciences · CN

Funding

Supplement: Exploring the biology of O-acetyl sialic acids using stable synthetic mimicsR01AI130684 · NIAID · UNIVERSITY OF CALIFORNIA AT DAVIS · PI CHEN, XI, VARKI, AJIT P · 2017 to 2020
$2.3M
Acquisition of a Q-Exactive Plus Mass SpectrometerS10OD025271 · OD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI JEWELL, WILLIAM T · 2019 to 2019
$486k
National Science Foundation DBI-0722538NIH HHS R01AI130684NIH HHS S10OD018913NIH HHS S10 OD025271NIH HHS S10OD025271
6 · The paper itself

Abstract

Carbohydrate-protein conjugates have diverse applications. They have been used clinically as vaccines against bacterial infection and have been developed for high-throughput assays to elucidate the ligand specificities of glycan-binding proteins (GBPs) and antibodies. Here, we report an effective process that combines highly efficient chemoenzymatic synthesis of carbohydrates, production of carbohydrate-bovine serum albumin (glycan-BSA) conjugates using a squarate linker, and convenient immobilization of the resulting neoglycoproteins on carboxylate-coated fluorescent magnetic beads for the development of a suspension multiplex array platform. A glycan-BSA-bead array containing BSA and 50 glycan-BSA conjugates with tuned glycan valency was generated. The binding profiles of six plant lectins with binding preference towards Gal and/or GalNAc, as well as human galectin-3 and galectin-8, were readily obtained. Our results provide useful information to understand the multivalent glycan-binding properties of human galectins. The neoglycoprotein-immobilized fluorescent magnetic bead suspension multiplex array is a robust and flexible platform for rapid analysis of glycan and GBP interactions and will find broad applications.

Indexed as

Blood ProteinsCarbohydrate ConformationCarbohydrate SequenceFluorescent DyesGalectinsGlycated Serum AlbuminGlycation End Products, AdvancedGlycoproteinsHumansImmobilized ProteinsMagnetic PhenomenaPlant LectinsPolysaccharidesProtein Array AnalysisProtein BindingRecombinant ProteinsBlood ProteinsFluorescent DyesGalectinsGlycated Serum AlbuminGlycation End Products, AdvancedGlycoproteinsImmobilized ProteinsLGALS3 protein, humanLGALS8 protein, humanPlant LectinsPolysaccharidesRecombinant ProteinsSerum AlbuminSerum Albumin, Bovinecarbohydrate-protein conjugategalectinglycan-binding proteinlectinmultiplex assay

Identifiers

PMID34684775
PMCPMC8541226
OpenAlexW3205138650

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.