Evidence map›Paper›PMID 34683222›Full record

ArticleMicromachines2021

Extending Single Cell Bioprinting from Femtosecond to Picosecond Laser Pulse Durations.

Jun Zhang, Yasemin Geiger, Florian Sotier, Sasa Djordjevic, Denitsa Docheva, Stefanie Sudhop, Hauke Clausen-Schaumann, Heinz P Huber

Abstract read
In one paragraph

Article in Micromachines, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Jun ZhangLasercenter, Department of Applied Sciences and Mechatronics, Munich University of Applied Sciences, Lothstrasse 34, 80335 Munich, Germany.
Yasemin GeigerLasercenter, Department of Applied Sciences and Mechatronics, Munich University of Applied Sciences, Lothstrasse 34, 80335 Munich, Germany.
Florian SotierInnoLas Photonics GmbH, Justus-von-Liebig-Ring 8, 82152 Krailling, Germany.ORCID 0000-0003-2039-4978
Sasa DjordjevicLasercenter, Department of Applied Sciences and Mechatronics, Munich University of Applied Sciences, Lothstrasse 34, 80335 Munich, Germany.
Denitsa DochevaExperimental Trauma Surgery, Department of Trauma Surgery, University Regensburg Medical Centre, Am Biopark 9, 93053 Regensburg, Germany.
Stefanie SudhopCenter for Applied Tissue Engineering and Regenerative Medicine CANTER, Munich University of Applied Sciences, Lothstrasse 34, 80335 Munich, Germany.ORCID 0000-0002-0838-9503
Hauke Clausen-SchaumannCenter for Applied Tissue Engineering and Regenerative Medicine CANTER, Munich University of Applied Sciences, Lothstrasse 34, 80335 Munich, Germany.ORCID 0000-0002-9413-0310
Heinz P HuberLasercenter, Department of Applied Sciences and Mechatronics, Munich University of Applied Sciences, Lothstrasse 34, 80335 Munich, Germany.ORCID 0000-0003-2444-9833

Funding

Achilles: Overcoming specific weakness in tendon biology to design advanced regenerative therapies" Proposal Nr. 810850 EU H2020-WIDESPREAD-05-2017-Twinning GrantBavarian Ministry of Science Forschungsschwerpunkt Angewandte Photonikthe Bavarian State Ministry of Science and the Arts Bavarian Academic Forum (BayWISS) - Doctoral Consortium "Gesundheit"
6 · The paper itself

Abstract

Femtosecond laser pulses have been successfully used for film-free single-cell bioprinting, enabling precise and efficient selection and positioning of individual mammalian cells from a complex cell mixture (based on morphology or fluorescence) onto a 2D target substrate or a 3D pre-processed scaffold. In order to evaluate the effects of higher pulse durations on the bioprinting process, we investigated cavitation bubble and jet dynamics in the femto- and picosecond regime. By increasing the laser pulse duration from 600 fs to 14.1 ps, less energy is deposited in the hydrogel for the cavitation bubble expansion, resulting in less kinetic energy for the jet propagation with a slower jet velocity. Under appropriate conditions, single cells can be reliably transferred with a cell survival rate after transfer above 95% through the entire pulse duration range. More cost efficient and compact laser sources with pulse durations in the picosecond range could be used for film-free bioprinting and single-cell transfer.

Indexed as

femtosecond laser-based bioprintingfilm-free LIFTlaser-induced forward transfer (LIFT)picosecond laser-based bioprintingsingle-cell bioprintingtissue engineering

Identifiers

PMID34683222
PMCPMC8538086

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.