Evidence map›Paper›PMID 34682231›Full record

ArticleJournal of fungi (Basel, Switzerland)2021

An Optimized and Efficient CRISPR/Cas9 System for the Endophytic Fungus

Xinran Xu, Runye Huang, Wen-Bing Yin

Open access · goldAbstract read
In one paragraph

Article in Journal of fungi (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 30 citations in OpenAlex.

  1. Review
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  6. Way to efficient microbial paclitaxel mass production.Synthetic and systems biotechnology · 2023
    Review
  7. Article
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  12. Molecules (Basel, Switzerland) · 2022
    Review
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Xinran XuState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.ORCID 0000-0003-3060-8554
Runye HuangState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
Wen-Bing YinState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.ORCID 0000-0002-9184-3198
Chinese Academy of Sciences · CN

Funding

Construction of the Registry and Database of Bioparts for Synthetic Biology ZSYS-016Key Research Program of Frontier Sciences, CAS ZDBS-LY-SM016National Key Research and Development Program of China 2020YFA0907801National Natural Science Foundation of China 31861133004
6 · The paper itself

Abstract

Endophytic fungi are emerging as attractive producers of natural products with diverse bioactivities and novel structures. However, difficulties in the genetic manipulation of endophytic fungi limit the search of novel secondary metabolites. In this study, we improved the polyethylene glycol (PEG)-mediated protoplast transformation method by introducing the CRISPR/Cas9 system into endophytic fungus

Indexed as

CRISPR/Cas9dual-locus genome editingendophytic fungigenetic manipulationPestalotiopsis fici

Identifiers

PMID34682231
PMCPMC8539907
OpenAlexW3203736764

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.