Evidence map›Paper›PMID 34681931›Full record

ArticleInternational journal of molecular sciences2021

Oleanolic Acid's Semisynthetic Derivatives HIMOXOL and Br-HIMOLID Show Proautophagic Potential and Inhibit Migration of HER2-Positive Breast Cancer Cells In Vitro.

Natalia Magdalena Lisiak, Izabela Lewicka, Mariusz Kaczmarek, Jacek Kujawski, Barbara Bednarczyk-Cwynar, Lucjusz Zaprutko, Blazej Rubis

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.6field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 11 citations in OpenAlex.

  1. Review
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  7. NOTCH Signaling in Osteosarcoma.Current issues in molecular biology · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Natalia Magdalena LisiakDepartment of Clinical Chemistry and Molecular Diagnostics, Poznan University of Medical Sciences, 49 Przybyszewskiego Str., 60-355 Poznan, Poland.
Izabela LewickaDepartment of Clinical Chemistry and Molecular Diagnostics, Poznan University of Medical Sciences, 49 Przybyszewskiego Str., 60-355 Poznan, Poland.
Mariusz KaczmarekDepartment of Cancer Immunology, Chair of Medical Biotechnology, Poznan University of Medical Sciences, Garbary 15 Str., 61-866 Poznan, Poland.ORCID 0000-0002-6282-3251
Jacek KujawskiDepartment of Organic Chemistry, Poznan University of Medical Sciences, 6 Grunwaldzka Str., 60-780 Poznan, Poland.ORCID 0000-0001-6279-4887
Barbara Bednarczyk-CwynarDepartment of Organic Chemistry, Poznan University of Medical Sciences, 6 Grunwaldzka Str., 60-780 Poznan, Poland.ORCID 0000-0003-2958-9106
Lucjusz ZaprutkoDepartment of Organic Chemistry, Poznan University of Medical Sciences, 6 Grunwaldzka Str., 60-780 Poznan, Poland.ORCID 0000-0003-1121-6272
Blazej RubisDepartment of Clinical Chemistry and Molecular Diagnostics, Poznan University of Medical Sciences, 49 Przybyszewskiego Str., 60-355 Poznan, Poland.ORCID 0000-0003-1730-0176
Poznan University of Medical Sciences · PL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Approximately 20-30% of the diagnosed breast cancers overexpress the human epidermal growth factor receptor 2 (HER2). This type of cancer is associated with a more aggressive phenotype; thus, there is a need for the discovery of new compounds that would improve the survival in HER2-positive breast cancer patients. It seems that one of the most promising therapeutic cancer strategies could be based on the biological activity of pentacyclic triterpenes' derivatives and the best-known representative of this group, oleanolic acid (OA). The biological activity of oleanolic acid and its two semisynthetic derivatives, methyl 3-hydroxyimino-11-oxoolean-12-en-28-oate (HIMOXOL) and 12α-bromo-3-hydroxyimonoolean-28→13-olide (Br-HIMOLID), was assessed in SK-BR-3 breast cancer cells (HER2-positive). Viability tests, cell cycle assessment, evaluation of apoptosis, autophagy, and adhesion/migration processes were performed using MTT, clonogenic, cytofluorometry, Western blot, and qPCR. Both derivatives revealed higher cytotoxicity in studied breast cancer cells than the maternal compound, OA. They also decreased cell viability, induced autophagy, and (when applied in sub-cytotoxic concentrations) decreased the migration of SK-BR-3 cells.This study is the first to report the cytostatic, proautophagic (mTOR/LC3/SQSTM/BECN1 pathway), and anti-migratory (integrin β1/FAK/paxillin pathway) activities of HIMOXOL and Br-HIMOLID in HER2-positive breast cancer cells.

Indexed as

AutophagyApoptosisBreast NeoplasmsCell CycleCell MovementCell ProliferationErb-b2 Receptor Tyrosine KinasesFemaleHumansIn Vitro TechniquesOleanolic AcidTumor Cells, Cultured12-bromo-3-hydroxyimonoolean-28-13-olideERBB2 protein, humanErb-b2 Receptor Tyrosine Kinasesmethyl 3-hydroxyimino-11-oxoolean-12-en-28-oateOleanolic Acidautophagybreast cancerBr-HIMOLIDHER2HIMOXOLmigrationoleanolic acidoleanolic acid derivatives

Identifiers

PMID34681931
PMCPMC8538366
OpenAlexW3205198576

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.