Evidence map›Paper›PMID 34681772›Full record

ArticleInternational journal of molecular sciences2021

Photophysical Properties of BADAN Revealed in the Study of GGBP Structural Transitions.

Alexander V Fonin, Sergey A Silonov, Iuliia A Antifeeva, Olga V Stepanenko, Olesya V Stepanenko, Anna S Fefilova, Olga I Povarova, Anastasia A Gavrilova, Irina M Kuznetsova, Konstantin K Turoverov

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PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Pitfalls of Using ANS Dye Under Molecular Crowding Conditions.International journal of molecular sciences · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Alexander V FoninLaboratory of Structural Dynamics, Stability and Folding of Proteins, Institute of Cytology, Russian Academy of Sciences, 4 Tikhoretsky Ave., 194064 Saint Petersburg, Russia.ORCID 0000-0003-4469-2531
Sergey A SilonovLaboratory of Structural Dynamics, Stability and Folding of Proteins, Institute of Cytology, Russian Academy of Sciences, 4 Tikhoretsky Ave., 194064 Saint Petersburg, Russia.ORCID 0000-0001-8714-6659
Iuliia A AntifeevaLaboratory of Structural Dynamics, Stability and Folding of Proteins, Institute of Cytology, Russian Academy of Sciences, 4 Tikhoretsky Ave., 194064 Saint Petersburg, Russia.
Olga V StepanenkoLaboratory of Structural Dynamics, Stability and Folding of Proteins, Institute of Cytology, Russian Academy of Sciences, 4 Tikhoretsky Ave., 194064 Saint Petersburg, Russia.ORCID 0000-0002-9145-3480
Olesya V StepanenkoLaboratory of Structural Dynamics, Stability and Folding of Proteins, Institute of Cytology, Russian Academy of Sciences, 4 Tikhoretsky Ave., 194064 Saint Petersburg, Russia.ORCID 0000-0003-3779-6711
Anna S FefilovaLaboratory of Structural Dynamics, Stability and Folding of Proteins, Institute of Cytology, Russian Academy of Sciences, 4 Tikhoretsky Ave., 194064 Saint Petersburg, Russia.ORCID 0000-0002-6927-4363
Olga I PovarovaLaboratory of Structural Dynamics, Stability and Folding of Proteins, Institute of Cytology, Russian Academy of Sciences, 4 Tikhoretsky Ave., 194064 Saint Petersburg, Russia.
Anastasia A GavrilovaLaboratory of Structural Dynamics, Stability and Folding of Proteins, Institute of Cytology, Russian Academy of Sciences, 4 Tikhoretsky Ave., 194064 Saint Petersburg, Russia.
Irina M KuznetsovaLaboratory of Structural Dynamics, Stability and Folding of Proteins, Institute of Cytology, Russian Academy of Sciences, 4 Tikhoretsky Ave., 194064 Saint Petersburg, Russia.ORCID 0000-0002-3336-4834
Konstantin K TuroverovLaboratory of Structural Dynamics, Stability and Folding of Proteins, Institute of Cytology, Russian Academy of Sciences, 4 Tikhoretsky Ave., 194064 Saint Petersburg, Russia.ORCID 0000-0002-6977-1896
Institute of Cytology · RUMoscow Institute of Physics and Technology · RU

Funding

The work is supported by the scholarship of the President of the Russian Federation SP-259.2019.4 (IAA)
6 · The paper itself

Abstract

The fluorescent dye BADAN (6-bromoacetyl-2-dimetylaminonaphtalene) is widely used in various fields of life sciences, however, the photophysical properties of BADAN are not fully understood. The study of the spectral properties of BADAN attached to a number of mutant forms of GGBP, as well as changes in its spectral characteristics during structural changes in proteins, allowed to shed light on the photophysical properties of BADAN. It was shown that spectral properties of BADAN are determined by at least one non-fluorescent and two fluorescent isomers with overlapping absorbing bands. It was found that BADAN fluorescence is determined by the unsolvated "PICT" (planar intramolecular charge transfer state) and solvated "TICT" (twisted intramolecular charge transfer state) excited states. While "TICT" state can be formed both as a result of the "PICT" state solvation and as a result of light absorption by the solvated ground state of the dye. BADAN fluorescence linked to GGBP/H152C apoform is quenched by Trp 183, but this effect is inhibited by glucose intercalation. New details of the changes in the spectral characteristics of BADAN during the unfolding of the protein apo and holoforms have been obtained.

Indexed as

2-NaphthylamineAmino Acid SubstitutionEscherichia coliEscherichia coli ProteinsFluorescenceFluorescent DyesMonosaccharide Transport ProteinsMutation, MissenseProtein ConformationSpectrometry, FluorescenceStructure-Activity Relationship2-Naphthylamine6-bromoacetyl-2-dimethylaminonaphthaleneEscherichia coli ProteinsFluorescent DyesmglB protein, E coliMonosaccharide Transport Proteinsapo- and holo-forms of GGBP (D-glucose/D-galactose-binding protein)BADAN (6-bromoacetyl-2-dimetylaminonaphtalene) spectroscopypathways of GGBP unfolding

Identifiers

PMID34681772
PMCPMC8540541
OpenAlexW3206578902

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.