ArticleGenes2021
Single-Cell RNA-Seq Revealed the Gene Expression Pattern during the In Vitro Maturation of Donkey Oocytes.
Article in Genes, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 16 citations in OpenAlex.
- Transcriptomic Analysis of Bovine Oocytes at GV and MII Stages and Dynamic Changes in Key Gene Expression Patterns.Biology · 2026Article
- Targeting Inhibin Enhances Wagyu Oocyte Competence and Embryo Quality: A Comparative Study of In Vivo Immunization and In Vitro Antibody Supplementation.Antioxidants (Basel, Switzerland) · 2026Article
- The Donkey Genome: From Evolutionary Insights to Sustainable Breeding Strategies.Animals : an open access journal from MDPI · 2025Review
- Cross-Species Insights intoAntioxidants (Basel, Switzerland) · 2025Article
- Revealing the Transcriptional and Metabolic Characteristics of Sebocytes Based on the Donkey Cell Transcriptome Atlas.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Current and Emerging Advanced Techniques for Breeding Donkeys and Mules.Animals : an open access journal from MDPI · 2025Review
- Unraveling the genetic and physiological potential of donkeys: insights from genomics, proteomics, and metabolomics approaches.Mammalian genome : official journal of the International Mammalian Genome Society · 2025Review
- Advances in single-cell transcriptomics in animal research.Journal of animal science and biotechnology · 2024Review
- A review of genetic resources and trends of omics applications in donkey research: focus on China.Frontiers in veterinary science · 2024Review
- Exploring Genetic Markers: Mitochondrial DNA and Genomic Screening for Biodiversity and Production Traits in Donkeys.Animals : an open access journal from MDPI · 2023Review
- Advances in Oocyte Maturation In Vivo and In Vitro in Mammals.International journal of molecular sciences · 2023Review
- Progesterone Promotes In Vitro Maturation of Domestic Dog Oocytes Leading to Successful Live Births.Life (Basel, Switzerland) · 2022Article
- Comparative Transcriptomics Uncover the Uniqueness of Oocyte Development in the Donkey.Frontiers in genetics · 2022Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Donkeys are an important domesticated animal, providing labor, meat, milk, and medicinal materials for humans. However, the donkey population is continuously declining and even at risk of extinction. The application of modern animal production technology, such as oocyte in vitro maturation, is a promising method to improve the donkey population. In this study, we explore the gene expression patterns of donkey germinal vesicle (GV) and in vitro matured metaphase II (MII) oocytes using single cell RNA-seq of the candidate genes along with the regulatory mechanisms that affect donkey oocyte maturation. We identified a total of 24,164 oocyte genes of which 9073 were significant differentially expressed in the GV and MII oocytes. Further Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that these genes were associated with the meiotic cell cycle, mitochondrion activity, and N-glycan biosynthesis, which might be the key genes and regulatory mechanisms affecting the maturation of donkey oocytes. Our study provides considerable understanding regarding the maturation of donkey oocytes and serves as a theoretical basis for improving the development of donkey oocytes, which could ultimately benefit the expansion of the donkey population and conservation of biodiversity and genetic resources.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.