Evidence map›Paper›PMID 34681026›Full record

ArticleGenes2021

Identification of Novel Endogenous Controls for qPCR Normalization in SK-BR-3 Breast Cancer Cell Line.

Nityanand Jain, Ingrida Mitre, Dina Nitisa, Valdis Pirsko, Inese Cakstina-Dzerve

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Genes, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Nityanand JainLaboratory of Molecular Genetics, Institute of Oncology, Faculty of Medicine, Riga Stradiņš University, Dzirciema Street 16, LV-1007 Riga, Latvia.ORCID 0000-0002-7918-7909
Ingrida MitreLaboratory of Molecular Genetics, Institute of Oncology, Faculty of Medicine, Riga Stradiņš University, Dzirciema Street 16, LV-1007 Riga, Latvia.ORCID 0000-0002-2889-3854
Dina NitisaLaboratory of Molecular Genetics, Institute of Oncology, Faculty of Medicine, Riga Stradiņš University, Dzirciema Street 16, LV-1007 Riga, Latvia.ORCID 0000-0002-3538-1187
Valdis PirskoLaboratory of Molecular Genetics, Institute of Oncology, Faculty of Medicine, Riga Stradiņš University, Dzirciema Street 16, LV-1007 Riga, Latvia.ORCID 0000-0002-9354-663X
Inese Cakstina-DzerveLaboratory of Molecular Genetics, Institute of Oncology, Faculty of Medicine, Riga Stradiņš University, Dzirciema Street 16, LV-1007 Riga, Latvia.ORCID 0000-0001-5589-0395

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Normalization of gene expression using internal controls or reference genes (RGs) has been the method of choice for standardizing the technical variations in reverse transcription quantitative polymerase chain reactions (RT-qPCR). Conventionally,

Indexed as

AlgorithmsApoptosis Regulatory ProteinsBreast NeoplasmsFemaleGene Expression Regulation, NeoplasticHSP90 Heat-Shock ProteinsHumansMembrane ProteinsProfilinsRibosomal ProteinsRNA-Binding ProteinsTranscriptomeApoptosis Regulatory ProteinsDAD1 protein, humanHSP90AB1 protein, humanHSP90 Heat-Shock ProteinsMembrane ProteinsPFN1 protein, humanProfilinsPUM1 protein, humanRibosomal ProteinsRNA-Binding Proteinsbreast cancer cell linegene expressionHER2 enrichedhypoxiareference genesRT-qPCRSK-BR-3

Identifiers

PMID34681026
PMCPMC8535678

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.