Evidence map›Paper›PMID 34680140›Full record

ArticleBiomolecules2021

Bucky Ball Is a Novel Zebrafish Vasa ATPase Activator.

Roshan Priyarangana Perera, Alaa Shaikhqasem, Nadia Rostam, Achim Dickmanns, Ralf Ficner, Kai Tittmann, Roland Dosch

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomolecules, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Roshan Priyarangana PereraDepartment of Developmental Biochemistry, University of Goettingen, 37077 Goettingen, Germany.ORCID 0000-0001-7239-7404
Alaa ShaikhqasemDepartment for Molecular Structural Biology, University of Goettingen, 37077 Goettingen, Germany.ORCID 0000-0002-5879-5585
Nadia RostamInstitute for Human Genetics, University of Goettingen, 37073 Goettingen, Germany.
Achim DickmannsDepartment for Molecular Structural Biology, University of Goettingen, 37077 Goettingen, Germany.
Ralf FicnerDepartment for Molecular Structural Biology, University of Goettingen, 37077 Goettingen, Germany.
Kai TittmannDepartment of Molecular Enzymology, University of Goettingen, 37077 Goettingen, Germany.ORCID 0000-0001-7891-7108
Roland DoschDepartment of Developmental Biochemistry, University of Goettingen, 37077 Goettingen, Germany.
University of Göttingen · DEMultiscale Bioimaging

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Many multicellular organisms specify germ cells during early embryogenesis by the inheritance of ribonucleoprotein (RNP) granules known as germplasm. However, the role of complex interactions of RNP granules during germ cell specification remains elusive. This study characterizes the interaction of RNP granules, Buc, and zebrafish Vasa (zfVasa) during germ cell specification. We identify a novel zfVasa-binding motif (Buc-VBM) in Buc and a Buc-binding motif (zfVasa-BBM) in zfVasa. Moreover, we show that Buc and zfVasa directly bind in vitro and that this interaction is independent of the RNA. Our circular dichroism spectroscopy data reveal that the intrinsically disordered Buc-VBM peptide forms alpha-helices in the presence of the solvent trifluoroethanol. Intriguingly, we further demonstrate that Buc-VBM enhances zfVasa ATPase activity, thereby annotating the first biochemical function of Buc as a zfVasa ATPase activator. Collectively, these results propose a model in which the activity of zfVasa is a central regulator of primordial germ cell (PGC) formation and is tightly controlled by the germplasm organizer Buc.

Indexed as

Adenosine TriphosphatasesAnimalsCytoplasmDEAD-box RNA HelicasesGerm CellsOocytesProtein BindingRibonucleoproteinsRNAZebrafishZebrafish ProteinsAdenosine Triphosphatasesbuc protein, zebrafishddx4 protein, zebrafishDEAD-box RNA HelicasesRibonucleoproteinsRNAZebrafish ProteinsATPase activityBucky ballDrosophilagermlinegermplasmOskarprimordial germ cellsVasazebrafish

Identifiers

PMID34680140
PMCPMC8533965
OpenAlexW3205942063

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.