Evidence map›Paper›PMID 34680080›Full record

ArticleBiomolecules2021

Characterization of Phosphorylation Status and Kinase Activity of Src Family Kinases Expressed in Cell-Based and Cell-Free Protein Expression Systems.

Emiko Kinoshita-Kikuta, Eiji Kinoshita, Misaki Suga, Mana Higashida, Yuka Yamane, Tomoka Nakamura, Tohru Koike

Open access · goldAbstract read
In one paragraph

Article in Biomolecules, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.5field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. Article
  3. Absolute quantification of fluorescent protein fusions by mass spectrometry.Protein science : a publication of the Protein Society · 2026
    Article
  4. Article
  5. Review
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Emiko Kinoshita-KikutaDepartment of Functional Molecular Science, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, Japan.ORCID 0000-0001-7355-1784
Eiji KinoshitaDepartment of Human Nutrition, Faculty of Human Sciences, Hiroshima Bunkyo University, Hiroshima 732-0295, Japan.ORCID 0000-0001-6179-6039
Misaki SugaSchool of Pharmaceutical Science, Hiroshima University, Hiroshima 734-8553, Japan.
Mana HigashidaSchool of Pharmaceutical Science, Hiroshima University, Hiroshima 734-8553, Japan.
Yuka YamaneSchool of Pharmaceutical Science, Hiroshima University, Hiroshima 734-8553, Japan.
Tomoka NakamuraSchool of Pharmaceutical Science, Hiroshima University, Hiroshima 734-8553, Japan.
Tohru KoikeDepartment of Functional Molecular Science, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, Japan.
Hiroshima University · JPHiroshima Bunkyo University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The production of heterologous proteins is an important procedure for biologists in basic and applied sciences. A variety of cell-based and cell-free protein expression systems are available to achieve this. The expression system must be selected carefully, especially for target proteins that require post-translational modifications. In this study, human Src family kinases were prepared using six different protein expression systems: 293 human embryonic kidney cells,

Indexed as

AnimalsCell-Free SystemEscherichia coliGene Expression RegulationGerm CellsHEK293 CellsHumansInsectaPhosphorylationRabbitsReticulocytessrc-Family KinasesTriticumsrc-Family Kinasesphosphorylationphos-tagprotein expression systemSrc family kinase

Identifiers

PMID34680080
PMCPMC8533471
OpenAlexW3203759988

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.