Evidence map›Paper›PMID 34677711›Full record

ArticleMolecular biology reports2021

Cloning and identification of a new repressor of 3,17β-Hydroxysteroid dehydrogenase of Comamonas testosteroni.

Weiqi Xie, Qin Xia, Ling Chen, Guangming Xiong, Yuwei Gao, Yuanhua Yu, Xiuxia He

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Article in Molecular biology reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Weiqi XieSchool of Life Science and Technology, Changchun University of Science and Technology, Changchun, 130022, China.
Qin XiaSchool of Life Science and Technology, Changchun University of Science and Technology, Changchun, 130022, China.
Ling ChenSchool of Life Science and Technology, Changchun University of Science and Technology, Changchun, 130022, China.
Guangming XiongInstitute of Toxicology and Pharmacology for Natural Scientists, University Medical School Schleswig-Holstein, 24103, Kiel, Germany.
Yuwei GaoInstitute of Military Veterinary Medicine, Academy of Military Medical Sciences, Changchun, 130122, China.
Yuanhua YuSchool of Life Science and Technology, Changchun University of Science and Technology, Changchun, 130022, China.
Xiuxia HeSchool of Life Science and Technology, Changchun University of Science and Technology, Changchun, 130022, China. hexx@cust.edu.cn.
Changchun University of Science and Technology · CNAcademy of Military Medical Sciences · CNUniversity of Lübeck · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

background3,17β-hydroxysteroid dehydrogenase (3,17β-HSD) is a key enzyme in the metabolic pathway for steroid compounds catabolism in Comamonas testosteroni. Tetracycline repressor (TetR) family, repressors existing in most microorganisms, may play key roles in regulating the expression of 3,17β-HSD. Previous reports showed that three tetR genes are located in the contig58 of C. testosteroni ATCC 11996 (GenBank: AHIL01000049.1), among which the first tetR gene encoded a potential repressor of 3,17β-HSD by sensing environmental signals. However, whether the other proposed tetR genes act as repressors of 3,17β-HSD are still unknown. METHODS AND

resultsIn the present study, we cloned the second tetR gene and analyzed the regulatory mechanism of the protein on 3,17β-HSD using electrophoretic mobility shift assay (EMSA), gold nanoparticles (AuNPs)-based assay, and loss-of-function analysis. The results showed that the second tetR gene was 660-bp, encoding a 26 kD protein, which could regulate the expression of 3,17β-HSD gene via binding to the conserved consensus sequences located 1100-bp upstream of the 3,17β-HSD gene. Furthermore, the mutant strain of C. testosteroni with the second tetR gene knocked-out mutant expresses good biological genetic stability, and the expression of 3,17β-HSD in the mutant strain is slightly higher than that in the wild type under testosterone induction.

conclusionsThe second tetR gene acts as a negative regulator in 3,17β-HSD expression, and the mutant has potential application in bioremediation of steroids contaminated environment.

Indexed as

Bacterial ProteinsCloning, MolecularComamonas testosteroniEnzyme InhibitorsTrans-Activators17-Hydroxysteroid DehydrogenasesRecombinant Proteins17-Hydroxysteroid Dehydrogenases3 (or 17)-beta-hydroxysteroid dehydrogenaseBacterial ProteinsEnzyme InhibitorsRecombinant ProteinsTrans-ActivatorsComamonas testosteroniElectrophoretic mobility shift assayGold nanoparticles (AuNPs)-based assaySteroid hormonesTetracycline repressor (TetR)

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PMID34677711
OpenAlexW3156253678

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