ArticleEnvironmental science and pollution research international2022
Production of hydroxytyrosol rich extract from Olea europaea leaf with enhanced biological activity using immobilized enzyme reactors.
Article in Environmental science and pollution research international, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
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- Green synthesis of magnetic bio-Graphene nanohybrid for the immobilization of hydrolytic enzymes towards sustainable bioconversion of cellulose.RSC advances · 2025Article
- Sustainable Bioethanol Production and Phenolic Compounds from Avocado Stone Biomass Based on Microwave Pretreatment.Foods (Basel, Switzerland) · 2025Article
- Network pharmacology of olive stem extract, UPLC-HR-QTOF-MS profiling and antiviral activities aligned with UN sustainable development goals.Scientific reports · 2025Article
- From Biosynthesis to Legislation: A Review of Hydroxytyrosol's Biological Functions and Safety.International journal of molecular sciences · 2025Review
- Enzymatic synthesis of Hydroxytyrosol from Oleuropein for valorization of an agricultural waste.Bioengineered · 2024Article
- A Study on the Regioselective Acetylation of Flavonoid Aglycons Catalyzed by Immobilized Lipases.Biomolecules · 2024Article
- Valorization of Olive Pomace Using Ultrasound-Assisted Extraction for Application in Active Packaging Films.International journal of molecular sciences · 2024Article
- Biosynthesis and Biotechnological Synthesis of Hydroxytyrosol.Foods (Basel, Switzerland) · 2024Review
- Laccase-Mediated Oxidation of Phenolic Compounds from Wine Lees Extract towards the Synthesis of Polymers with Potential Applications in Food Packaging.Biomolecules · 2024Article
- Development of Low-Density Polyethylene Films Coated with Phenolic Substances for Prolonged Bioactivity.Polymers · 2023Article
- Enzymatic Modification of Pomace Olive Oil with Natural Antioxidants: Effect on Oxidative Stability.Biomolecules · 2023Article
- Antioxidant and Anti-Diabetic Properties of Olive (Antioxidants (Basel, Switzerland) · 2023Article
- Designing Antioxidant and Antimicrobial Polyethylene Films with Bioactive Compounds/Clay Nanohybrids for Potential Packaging Applications.Molecules (Basel, Switzerland) · 2023Article
- Olive Leaf as a Source of Antibacterial Compounds Active against Antibiotic-Resistant Strains ofAntibiotics (Basel, Switzerland) · 2022Article
- Assessing environmental impacts through innovative solutions.Environmental science and pollution research international · 2022Article
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Authors and funding
9 authors at 1 institution in 1 country.
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Abstract
As olive leaves constitute the main by-product of the olive oil industry with important environmental and economic impact, there is an increasing demand for its valorization. In the present work, we report the development and application of immobilized enzyme batch bioreactors for the chemo-enzymatic treatment of an aqueous Olea europaea leaf extract rich in oleuropein to produce an extract enriched in hydroxytyrosol and other oleuropein hydrolysis products. To this end, a robust biocatalyst was developed through the immobilization of β-glucosidase on chitosan-coated magnetic beads which exhibited high hydrolytic stability after 240 h of incubation at 37 °C. The biocatalyst was successfully used in both a rotating bed-reactor and a stir-tank reactor for the modification of the olive leaf extract leading to high conversion yields of oleuropein (exceeding 90%), while an up to 2.5 times enrichment in hydroxytyrosol was achieved. Over 20 phenolic compounds (from different classes of phytochemicals such as flavonoids, secoiridoids, and their derivatives) were identified, in the extract before and after its modification through various chromatographic and spectroscopic techniques. Finally, the biological activity of both extracts was evaluated. Compared to the non-modified extract, the modified one demonstrated 20% higher antioxidant activity, seven-fold higher antibacterial activity, and enhanced cytotoxicity against leiomyosarcoma cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.