Evidence map›Paper›PMID 34675503›Full record

ArticleInternational journal of chronic obstructive pulmonary disease

Human Adipose-Derived Mesenchymal Stem Cells Ameliorate Elastase-Induced Emphysema in Mice by Mesenchymal-Epithelial Transition.

Nobuhiro Fujioka, Masahiro Kitabatake, Noriko Ouji-Sageshima, Takahiro Ibaraki, Makiko Kumamoto, Yukio Fujita, Shigeto Hontsu, Motoo Yamauchi, Masanori Yoshikawa, Shigeo Muro and 1 more

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In one paragraph

Article in International journal of chronic obstructive pulmonary disease. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.1field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
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  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Nobuhiro FujiokaDepartment of Respiratory Medicine, Nara Medical University, Kashihara, Nara, Japan.
Masahiro KitabatakeDepartment of Immunology, Nara Medical University, Kashihara, Nara, Japan.
Noriko Ouji-SageshimaDepartment of Immunology, Nara Medical University, Kashihara, Nara, Japan.
Takahiro IbarakiDepartment of Respiratory Medicine, Nara Medical University, Kashihara, Nara, Japan.
Makiko KumamotoDepartment of Respiratory Medicine, Nara Medical University, Kashihara, Nara, Japan.
Yukio FujitaDepartment of Respiratory Medicine, Nara Medical University, Kashihara, Nara, Japan.
Shigeto HontsuDepartment of Respiratory Medicine, Nara Medical University, Kashihara, Nara, Japan.
Motoo YamauchiDepartment of Respiratory Medicine, Nara Medical University, Kashihara, Nara, Japan.
Masanori YoshikawaDepartment of Respiratory Medicine, Nara Medical University, Kashihara, Nara, Japan.
Shigeo MuroDepartment of Respiratory Medicine, Nara Medical University, Kashihara, Nara, Japan.ORCID 0000-0001-7452-9191
Toshihiro ItoDepartment of Immunology, Nara Medical University, Kashihara, Nara, Japan.
Nara Medical University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeChronic obstructive pulmonary disease (COPD) is a worldwide problem because of its high prevalence and mortality. However, there is no fundamental treatment to ameliorate their pathological change in COPD lung. Recently, adipose-derived mesenchymal stem cells (ADSCs) have attracted attention in the field of regenerative medicine to repair damaged organs. Moreover, their utility in treating respiratory diseases has been reported in some animal models. However, the detailed mechanism by which ADSCs improve chronic respiratory diseases, including COPD, remains to be elucidated. We examined whether human ADSCs (hADSCs) ameliorated elastase-induced emphysema and whether hADSCs differentiated into alveolar epithelial cells in a murine model of COPD.

methodsFemale SCID-beige mice (6 weeks old) were divided into the following four groups according to whether they received an intratracheal injection of phosphate-buffered saline or porcine pancreatic elastase, and whether they received an intravenous injection of saline or hADSCs 3 days after intratracheal injection; Control group, hADSC group, Elastase group, and Elastase-hADSC group. We evaluated the lung function, assessed histological changes, and compared gene expression between hADSCs isolated from the lung of Elastase-hADSC group and naïve hADSCs 28 days after saline or elastase administration.

resultshADSCs improved the pathogenesis of COPD, including the mean linear intercept and forced expiratory volume, in an elastase-induced emphysema model in mice. Furthermore, hADSCs were observed in the lungs of elastase-treated mice at 25 days after administration. These cells expressed genes related to mesenchymal-epithelial transition and surface markers of alveolar epithelial cells, such as TTF-1, β-catenin, and E-cadherin.

conclusionhADSCs have the potential to improve the pathogenesis of COPD by differentiating into alveolar epithelial cells by mesenchymal-epithelial transition.

Indexed as

EmphysemaMesenchymal Stem CellsPulmonary Disease, Chronic ObstructivePulmonary EmphysemaAnimalsDisease Models, AnimalFemaleHumansMiceMice, Inbred C57BLMice, SCIDPancreatic ElastaseSwinePancreatic Elastaseadipose derived mesenchymal stem cellchronic obstructive pulmonary diseasemesenchymal–epithelial transitionpulmonary function test

Identifiers

PMID34675503
PMCPMC8517419
OpenAlexW3203342316

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.