ArticleScientific reports2021
Comparison of bacteria disintegration methods and their influence on data analysis in metabolomics.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Bacterial Espresso: Evaluating Accelerated Solvent Extraction for Selected Metabolite Recovery fromJournal of natural products · 2026Article
- Infrared Spectroscopic Profiling of Volatile Metabolites from Uropathogenic Bacteria: Basic Investigations toward Rapid UTI Diagnostics.Analytical chemistry · 2025Article
- Anandamide Alters Glycolytic Activity inInternational journal of molecular sciences · 2025Article
- The MDA-MB-231 Breast Cancer Cell Secretomes Modify Metabolomes ofInternational journal of molecular sciences · 2025Article
- The influence of benzene on the composition, diversity and performance of the anodic bacterial community in glucose-fed microbial fuel cells.Frontiers in microbiology · 2024Article
- Evaluation of cell disruption methods for protein and coenzyme Q10 quantification in purple non-sulfur bacteria.Frontiers in microbiology · 2024Article
- MetaboLINK is a novel algorithm for unveiling cell-specific metabolic pathways in longitudinal datasets.Frontiers in neuroscience · 2024Article
- Decoding the metabolic response ofProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Sample Preparation in Microbial Metabolomics: Advances and Challenges.Advances in experimental medicine and biology · 2023Article
- Advances in Microbial NMR Metabolomics.Advances in experimental medicine and biology · 2023Article
- Identification of metabolite extraction method for targeted exploration of antimicrobial resistance associated metabolites of Klebsiella pneumoniae.Scientific reports · 2022Article
- Bacterial Metabolomics: Sample Preparation Methods.Biochemistry research international · 2022Review
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Authors and funding
4 authors.
Funding
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Abstract
Metabolomic experiments usually contain many different steps, each of which can strongly influence the obtained results. In this work, metabolic analyses of six bacterial strains were performed in light of three different bacterial cell disintegration methods. Three strains were gram-negative (Pseudomonas aeruginosa, Escherichia coli, and Klebsiella pneumoniae), and three were gram-positive (Corynebacterium glutamicum, Bacillus cereus, and Enterococcus faecalis). For extraction, the methanol-water extraction method (1:1) was chosen. To compare the efficiency of different cell disintegration methods, sonication, sand mill, and tissue lyser were used. For bacterial extract metabolite analysis,
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