Evidence map›Paper›PMID 34674019›Full record

ArticlePediatric surgery international2022

Differential expression of miRNA in histological subtype of Wilms tumor.

Disha Kakkar, Saumyaranjan Mallick, Aijaz Ahmad, Ansh Goswami, Sandeep Agarwala, Aditya Kumar Gupta, V Sreenivas, Sameer Bakhshi, K Devasenathipathy, Sandeep Mathur and 3 more

Abstract read
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Article in Pediatric surgery international, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. MicroRNAs in idiopathic childhood nephrotic syndrome.Clinical and experimental nephrology · 2025
    Article
  2. micro-RNA 451-a as a Circulating Biomarker for Neuroblastoma.MicroRNA (Shariqah, United Arab Emirates) · 2025
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Disha Kakkar *Department of Pathology, All India Institute of Medical Sciences, New Delhi, 110029, India.
Saumyaranjan Mallick *Department of Pathology, All India Institute of Medical Sciences, New Delhi, 110029, India.
Aijaz Ahmad *Department of Pathology, All India Institute of Medical Sciences, New Delhi, 110029, India.
Ansh GoswamiDepartment of Pathology, All India Institute of Medical Sciences, New Delhi, 110029, India.
Sandeep AgarwalaDepartment of Pediatric Surgery, All India Institute of Medical Sciences, New Delhi, 110029, India.
Aditya Kumar GuptaDepartment of Paediatrics, All India Institute of Medical Sciences, New Delhi, 110029, India.
V SreenivasDepartment of Biostatistics, All India Institute of Medical Sciences, New Delhi, 110029, India.
Sameer BakhshiDepartment of Medical Oncology, All India Institute of Medical Sciences, New Delhi, 110029, India.
K DevasenathipathyDepartment of Radiology, All India Institute of Medical Sciences, New Delhi, 110029, India.
Sandeep MathurDepartment of Pathology, All India Institute of Medical Sciences, New Delhi, 110029, India.
Deepali JainDepartment of Pathology, All India Institute of Medical Sciences, New Delhi, 110029, India.
Rachana SethDepartment of Paediatrics, All India Institute of Medical Sciences, New Delhi, 110029, India.
Venkateswaran K IyerDepartment of Pathology, All India Institute of Medical Sciences, New Delhi, 110029, India. iyervenkat4@gmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionWilms tumor is the most common renal malignancy in children and difficult to differentiate from other paediatric abdominal tumors radiologically, necessitating an invasive procedure for diagnosis. Previous studies have shown the potential role of miRNA as biomarkers for diagnosis, histological subtyping and prognosis. In this study, we are exploring the role of miRNA in the histological subtyping of Wilms tumor in the Indian population. MATERIALS AND

methodsA total of 15 cases of Wilms tumor were evaluated for global miRNA expression analysis by microarray. Total RNA was extracted from fresh frozen tumor and miRNA expression analysis was performed using Agilent platform. Unsupervised clustering was done to analyse the data.

resultsUsing unpaired student T test, top 10 significantly differentially expressed miRNA were selected which could differentiate among different histological subtypes by unsupervised hierarchical clustering and principal component analysis. The presence of necrosis, heterologous differentiation led to change in miRNA expression profile and led to a distinct cluster formation.

conclusionsA panel of 5 miRNAs (miR1, 133b, 299-3p, 499a-5p, 491-3p) could differentiate among different histological subtypes of Wilms tumor, thus avoiding an invasive procedure in children, however, further confirmation using real time PCR analysis will be needed.

Indexed as

Kidney NeoplasmsMicroRNAsWilms TumorBiomarkers, TumorChildGene Expression ProfilingGene Expression Regulation, NeoplasticHumansPrognosisReal-Time Polymerase Chain ReactionBiomarkers, TumorMicroRNAsHistologymiRNA expressionPrognosisWilms tumor

Identifiers

PMID34674019

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