ArticleFrontiers in cellular and infection microbiology2021
A Microbial World: Could Metagenomic Next-Generation Sequencing Be Involved in Acute Respiratory Failure?
Article in Frontiers in cellular and infection microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- The diagnostic value of metagenomic next-generation sequencing versus traditional microbiological testing in native pyogenic spinal infections: A systematic review and meta-analysis.North American Spine Society journal · 2026Review
- HMGB1 and metabolic pathways in Acinetobacter baumannii pneumonia: an associative study.BMC infectious diseases · 2025Article
- Changes of respiratory microbiota associated with prognosis in pulmonary infection patients with invasive mechanical ventilation-supported respiratory failure.Annals of medicine · 2025Article
- HTS and PCR Methods Are the Most Used in the Diagnosis of Aspergillosis: Advantages over Other Molecular Methods.Journal of fungi (Basel, Switzerland) · 2025Review
- Severe pneumonia due to concurrent Legionella pneumophila and Acinetobacter baumannii infections: a case report.BMC pulmonary medicine · 2025Article
- Diagnosis, treatment, and monitoring of cytomegalovirus pneumonia in a hematopoietic stem cell transplantation child.Frontiers in cellular and infection microbiology · 2025Article
- High utility of bronchoalveolar lavage fluid metagenomic next-generation sequencing approach for etiological diagnosis of pneumonia.BMC infectious diseases · 2024Article
- Comparative analysis of metagenomic and targeted next-generation sequencing for pathogens diagnosis in bronchoalveolar lavage fluid specimens.Frontiers in cellular and infection microbiology · 2024Article
- Higher diagnostic value of metagenomic next-generation sequencing in acute infection than chronic infection: a multicenter retrospective study.Frontiers in microbiology · 2024Article
- Acarbose reduces Pseudomonas aeruginosa respiratory tract infection in type 2 diabetic mice.Respiratory research · 2023Article
- The gut microbiota pathway mechanisms of diabetes.AMB Express · 2023Review
- Deciphering Microbiota of Acute Upper Respiratory Infections: A Comparative Analysis of PCR and mNGS Methods for Lower Respiratory Trafficking Potential.Advances in respiratory medicine · 2023Article
- Diagnostic accuracy and microbial profiles of tuberculous pleurisy: a comparative study of metagenomic next generation sequencing and GeneXpertFrontiers in cellular and infection microbiology · 2023Article
- Multiplex PCR-based next generation sequencing as a novel, targeted and accurate molecular approach for periprosthetic joint infection diagnosis.Frontiers in microbiology · 2023Article
- Clinical features and diagnostic value of metagenomic next -generation sequencing in five cases of non-HIV relatedFrontiers in cellular and infection microbiology · 2023Article
- Application value of blood metagenomic next-generation sequencing in patients with connective tissue diseases.Frontiers in immunology · 2022Article
- Significance of pleural effusion detected by metagenomic next-generation sequencing in the diagnosis of aspiration pneumonia.Frontiers in cellular and infection microbiology · 2022Article
- Comparing the application of mNGS after combined pneumonia in hematologic patients receiving hematopoietic stem cell transplantation and chemotherapy: A retrospective analysis.Frontiers in cellular and infection microbiology · 2022Article
- Metagenomic next-generation sequencing: A promising tool for diagnosis and treatment of suspected pneumonia in rheumatic patients with acute respiratory failure: Retrospective cohort study.Frontiers in cellular and infection microbiology · 2022Article
- Application of Next-Generation Sequencing in Infections After Allogeneic Haematopoietic Stem Cell Transplantation: A Retrospective Study.Frontiers in cellular and infection microbiology · 2022Article
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: The usefulness of metagenomic next-generation sequencing (mNGS) in identifying pathogens is being investigated. We aimed to compare the power of microbial identification between mNGS and various methods in patients with acute respiratory failure. Methods: We reviewed 130 patients with respiratory failure, and 184 specimens including blood, bronchoalveolar lavage fluid (BALF), sputum, pleural effusion, ascitic fluid, and urine were tested by mNGS and conventional methods (culture, PCR). We also enrolled 13 patients to evaluate the power of mNGS and pathogen targets NGS (ptNGS) in microbial identifications. Clinical features and microbes detected were analyzed. Results: mNGS outperformed the conventional method in the positive detection rate of Conclusion: mNGS outperformed the conventional method in the detection of MTB, bacteria, fungi, mycoplasma, and virus. Orotracheal intubation, T2DM, immunosuppression, and inflammatory markers were associated with a higher detection rate of bacteria, fungi, and virus by mNGS. In addition, ptNGS results were consistent with the detection of abundant bacteria, fungi, and mycoplasma in our specimens.
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