ArticleThe Journal of surgical research2022
Optimization of Ex Vivo Machine Perfusion and Transplantation of Vascularized Composite Allografts.
Article in The Journal of surgical research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 2 of them syntheses that pooled it.
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Who cites it
17 citing papers in PubMed, 2 syntheses or guidelines pooled it, 27 citations in OpenAlex.
- A meta-analysis of perfusion parameters affecting weight gain in ex vivo perfusion.Artificial organs · 2025Pooled it
- Transplant international : official journal of the European Society for Organ Transplantation · 2025Pooled it
- Dual acellular subnormothermic machine perfusion for enhanced uterus preservation in a swine model.Acta obstetricia et gynecologica Scandinavica · 2026Article
- Development of a Swine Whole Eye Transplant Ex Vivo Perfusion Protocol.Artificial organs · 2026Article
- 24-hourFrontiers in transplantation · 2026Article
- Laryngeal transplantation: from experimental feasibility to multidisciplinary clinical reality.Frontiers in surgery · 2026Review
- Development of a 24-h Preservation Protocol of Forearm Vascularized Composite Allotransplants in Nonhuman Primates Using Subnormothermic Machine Perfusion.Transplantation direct · 2025Article
- Ex Vivo Preservation of Porcine Vascularized Composite Soft Tissue Allografts.Artificial organs · 2025Article
- A Guide to the Implementation and Design of Ex Vivo Perfusion Machines for Vascularized Composite Allotransplantation.Plastic and reconstructive surgery. Global open · 2024Article
- Supercooling preservation of vascularized composite allografts through CPA optimization, thermal tracking, and stepwise loading techniques.Scientific reports · 2024Article
- VCA supercooling in a swine partial hindlimb model.Scientific reports · 2024Article
- Towards Optimizing Sub-Normothermic Machine Perfusion in Fasciocutaneous Flaps: A Large Animal Study.Bioengineering (Basel, Switzerland) · 2023Article
- Weight gain is an early indicator of injury in ex vivo normothermic limb perfusion (EVNLP).Artificial organs · 2023Article
- Review of machine perfusion studies in vascularized composite allotransplant preservation.Frontiers in transplantation · 2023Review
- Development of a rat forelimb vascularized composite allograft (VCA) perfusion protocol.PloS one · 2023Article
- Improving the ischemia-reperfusion injury in vascularized composite allotransplantation: Clinical experience and experimental implications.Frontiers in immunology · 2022Review
- The future role of mitochondrial drugs in vascularized composite allotransplantation: A short review.Cell transplantationReview
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 3 countries.
Funding
Abstract
backgroundMachine perfusion is gaining interest as an efficient method of tissue preservation of Vascularized Composite Allografts (VCA). The aim of this study was to develop a protocol for ex vivo subnormothermic oxygenated machine perfusion (SNMP) on rodent hindlimbs and to validate our protocol in a heterotopic hindlimb transplant model.
methodsIn this optimization study we compared three different solutions during 6 h of SNMP (n = 4 per group). Ten control limbs were stored in a preservation solution on Static Cold Storage [SCS]). During SNMP we monitored arterial flowrate, lactate levels, and edema. After SNMP, muscle biopsies were taken for histology examination, and energy charge analysis. We validated the best perfusion protocol in a heterotopic limb transplantation model with 30-d follow up (n = 13). As controls, we transplanted untreated limbs (n = 5) and hindlimbs preserved with either 6 or 24 h of SCS (n = 4 and n = 5).
resultsDuring SNMP, arterial outflow increased, and lactate clearance decreased in all groups. Total edema was significantly lower in the HBOC-201 group compared to the BSA group (P = 0.005), 4.9 (4.3-6.1) versus 48.8 (39.1-53.2) percentage, but not to the BSA + PEG group (P = 0.19). Energy charge levels of SCS controls decreased 4-fold compared to limbs perfused with acellular oxygen carrier HBOC-201, 0.10 (0.07-0.17) versus 0.46 (0.42-0.49) respectively (P = 0.002).
conclusionsSix hours ex vivo SNMP of rodent hindlimbs using an acellular oxygen carrier HBOC-201 results in superior tissue preservation compared to conventional SCS.
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Registered trials
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